DIRECT EVIDENCE THAT THE GENE-PRODUCT OF THE HUMAN CHROMOSOME-21 LOCUS, IFRC, IS THE INTERFERON-ALPHA RECEPTOR
DIRECT EVIDENCE THAT THE GENE-PRODUCT OF THE HUMAN CHROMOSOME-21 LOCUS, IFRC, IS THE INTERFERON-ALPHA RECEPTOR
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DOI：
10.1016/0006-291x(82)90629-5
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
BAGLIONI, C
中科院分区：
文献类型：
--
作者：
EPSTEIN, CJ;MCMANUS, NH;BAGLIONI, C
基于间接功能和抗体阻断证据，认为人类21号染色体上的基因IFRC编码干扰素受体。为了获得该结论的直接证据，测定了125 I-HuIFN [人干扰素]-α A与染色体21的成纤维细胞二倍体和非整倍体的匹配组的特异性结合。虽然IFN-α的解离常数对于三体和二倍体细胞都是相同的，三体细胞结合更多的IFN-α。而单体细胞比二倍体细胞少。当将所有测定的数据合并时，21号单体：二倍体：21号三体结合比为0.5：0.8：1.5，与基于对编码产物的严格基因剂量考虑而预期的0.5：1.0：1.5的值非常一致21号染色体。由IFRC确定并被阻断IFN作用的抗体识别的染色体21基因产物确实是HIF-α的特异性细胞表面受体。
The gene, IFRC, on human chromosome 21 was considered on the basis of indirect functional and antibody blocking evidence, to code for an interferon receptor. To obtain direct evidence for this conclusion, the specific binding of 125I-HuIFN[human-interferon]-.alpha.A to matched sets of fibroblasts diploid and aneuploid for chromosome 21 was determined. Although the dissociation constant for IFN-.alpha. is the same for both trisomic and diploid cells, trisomic cells bind more IFN-.alpha. and monosomic cells less than do diploid cells. When the data for all determinations are combined, the monosomy 21:diploid:trisomy 21 binding ratios are 0.5:0.8:1.5, in good agreement with the values of 0.5:1.0:1.5 expected on the basis of strict gene dosage considerations for a product coded for by chromosome 21. The chromosome 21 gene product determined by IFRC and recognized by antibodies which block IFN action is truly a specific cell surface receptor for HIF-.alpha.