Potential for false positive HIV test results with the serial rapid HIV testing algorithm.
Potential for false positive HIV test results with the serial rapid HIV testing algorithm.
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DOI：
10.1186/1756-0500-5-154
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发表时间：
2012-03-19
影响因子：
1.8
通讯作者：
Wanyenze, Rhoda K
中科院分区：
文献类型：
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作者：
Baveewo, Steven;Kamya, Moses R;Wanyenze, Rhoda K
背景技术背景：快速HIV检测提供当天结果，并广泛用于人员和实验室基础设施有限的地区的HIV检测项目。乌干达卫生部目前建议使用Determine、STAT-PAK和Uni-Gold系列快速检测算法诊断艾滋病毒感染。使用该算法，Determine检测呈阳性、STAT-PAK检测呈阴性且Uni-Gold检测呈阳性的个体报告为HIV阳性。我们进行了进一步的测试，这个亚组的样本使用定性DNA PCR，以评估假阳性测试的可能性，在这种situation.RESULTS：在3388人进行了测试，984人是HIV阳性的两个连续的测试，和29被认为是阳性的决胜局（阳性确定，阴性的STAT-PAK，和阳性的Uni-Gold）。然而，当使用定性DNA PCR对29个样品进行进一步检测时，14个（48.2%）是HIV阴性。结论：虽然这项研究的主要目的不是为了评估快速HIV检测的有效性，因此只有一个子集的样本进行了重新测试，结果显示假阳性HIV结果在子集的个人谁测试阳性时，一个决胜局测试是在系列测试中使用。这些发现强调了需要对这类人进行确认性测试。
BACKGROUND: Rapid HIV tests provide same-day results and are widely used in HIV testing programs in areas with limited personnel and laboratory infrastructure. The Uganda Ministry of Health currently recommends the serial rapid testing algorithm with Determine, STAT-PAK, and Uni-Gold for diagnosis of HIV infection. Using this algorithm, individuals who test positive on Determine, negative to STAT-PAK and positive to Uni-Gold are reported as HIV positive. We conducted further testing on this subgroup of samples using qualitative DNA PCR to assess the potential for false positive tests in this situation.RESULTS: Of the 3388 individuals who were tested, 984 were HIV positive on two consecutive tests, and 29 were considered positive by a tiebreaker (positive on Determine, negative on STAT-PAK, and positive on Uni-Gold). However, when the 29 samples were further tested using qualitative DNA PCR, 14 (48.2%) were HIV negative.CONCLUSION: Although this study was not primarily designed to assess the validity of rapid HIV tests and thus only a subset of the samples were retested, the findings show a potential for false positive HIV results in the subset of individuals who test positive when a tiebreaker test is used in serial testing. These findings highlight a need for confirmatory testing for this category of individuals.