Influence of thylakoid membrane lipids on the structure of aggregated light-harvesting complexes of the diatom Thalassiosira pseudonana and the green alga Mantoniella squamata.
Influence of thylakoid membrane lipids on the structure of aggregated light-harvesting complexes of the diatom Thalassiosira pseudonana and the green alga Mantoniella squamata.
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DOI：
10.1111/ppl.12565
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发表时间：
2017-07
影响因子：
6.4
通讯作者：
Susann Schaller-Laudel;D. Latowski;M. Jemioła-Rzemińska;K. Strzałka;Sebastian Daum;K. Bacia;C. Wilhelm;R. Goss
中科院分区：
文献类型：
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作者：
Susann Schaller-Laudel;D. Latowski;M. Jemioła-Rzemińska;K. Strzałka;Sebastian Daum;K. Bacia;C. Wilhelm;R. Goss
研究了类囊体膜脂单半乳糖二酰甘油(MGDG)、双半乳糖二甘油(DGDG)、磺基喹诺酮二甘油(SQDG)和磷脂酰甘油(PG)对两种藻类捕光复合体(LHC)结构的影响。与高等植物的类囊体膜以富含中性半乳脂MGDG和DGDG为特征不同，绿藻Mantoniella Squamata和中心硅藻Thalassiosira Pusonana都含有高含量的负电荷脂类SQDG和PG。藻类囊体没有表现出高等植物典型的基粒-基质分化，而是呈规则排列。以Triton X-100为洗涤剂，采用连续阳离子沉淀法制备了假单胞藻的岩藻黄质叶绿素蛋白(FCP)复合体和鳞毛藻的LHC(MLHC)，分析了膜脂对膜脂的影响。通过这种方法，可以以聚集状态分离具有较少数量的相关脂类的LHC。77K荧光光谱和光子相关光谱的结果表明，中性半乳糖脂和带负电荷的脂类都不能显著改变FCP和MLHC的聚集状态。这与高等植物相反，在高等植物中，SQDG和PG导致LHCII强烈解聚，而MGDG和DGDG则诱导形成大的大聚集体。结果表明，与富含中性脂质的类囊体膜中的LHC相比，整合到类囊体膜中的LHC具有较高的负电荷脂含量和规则的排列方式，对脂质诱导的结构变化不那么敏感。
The study investigated the effect of the thylakoid membrane lipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG) on the structure of two algal light-harvesting complexes (LHCs). In contrast to higher plants whose thylakoid membranes are characterized by an enrichment of the neutral galactolipids MGDG and DGDG, both the green alga Mantoniella squamata and the centric diatom Thalassiosira pseudonana contain membranes with a high content of the negatively charged lipids SQDG and PG. The algal thylakoids do not show the typical grana-stroma differentiation of higher plants but a regular arrangement. To analyze the effect of the membrane lipids, the fucoxanthin chlorophyll protein (FCP) complex of T. pseudonana and the LHC of M. squamata (MLHC) were prepared by successive cation precipitation using Triton X-100 as detergent. With this method, it is possible to isolate LHCs with a reduced amount of associated lipids in an aggregated state. The results from 77 K fluorescence and photon correlation spectroscopy show that neither the neutral galactolipids nor the negatively charged lipids are able to significantly alter the aggregation state of the FCP or the MLHC. This is in contrast to higher plants where SQDG and PG lead to a strong disaggregation of the LHCII whereas MGDG and DGDG induce the formation of large macroaggregates. The results indicate that LHCs which are integrated into thylakoid membranes with a high amount of negatively charged lipids and a regular arrangement are less sensitive to lipid-induced structural alterations than their counterparts in membranes enriched in neutral lipids with a grana-stroma differentiation.