CHARACTERIZATION OF CALRETININ-IMMUNOREACTIVE STRUCTURES IN THE STRIATUM OF THE RAT
CHARACTERIZATION OF CALRETININ-IMMUNOREACTIVE STRUCTURES IN THE STRIATUM OF THE RAT
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DOI：
10.1016/0006-8993(93)90866-l
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发表时间：
1993-04-23
期刊：
影响因子：
2.9
通讯作者：
BOLAM, JP
中科院分区：
文献类型：
--
作者：
BENNETT, BD;BOLAM, JP
先前的观察表明，纹状体中含有一群对钙视网膜蛋白具有免疫反应性的神经元。为了从形态学上表征这些神经元，对大鼠纹状体的切片进行免疫染色以揭示钙视网膜蛋白，并在光镜和电镜水平上进行检查。纹状体包含一个小的人口calretinin免疫反应神经元，这是中等大小（9-17妈妈），并拥有一些多刺的，很少分支树突逐渐变细，成为非常薄的过程中，他们的最远端部分。虽然calretinin免疫反应神经元均匀分布在额状面，有一个显着的rostrocaudal梯度与更大的细胞密度比在纹状体的尾部。在超微结构水平上，calretinin免疫反应神经元被认为拥有一个缩进的核，并接受突触输入至少三种类型的终扣。除了calretinin免疫反应神经元，纹状体也包含轴突和终端boutons显示calretinin的免疫反应。至少有两种类型的免疫反应性终端被确定，那些形成对称的突触专业化和那些形成不对称的突触专业化。大约50%的形成不对称的接触与棘和30%的形成对称的突触接触与树突轴。在试图进一步的化学特性的含钙视黄蛋白的神经元，双包埋前的钙视黄蛋白和小白蛋白或胆碱乙酰转移酶的免疫细胞化学进行和钙视黄蛋白的免疫细胞化学与NADPH-黄递酶的组织化学相结合。这些双染色切片的分析表明，人口的钙视蛋白免疫反应神经元是不同的神经元含有小清蛋白，胆碱乙酰转移酶或NADPH-黄递酶的人口。结论是：（1）在分布、形态、化学、超微结构和传入突触输入的基础上，calretinin免疫反应神经元不同于先前在纹状体中识别的主要类别的神经元;（2）calretinin免疫反应终末是异质的，可能来自局部含calretinin的神经元和可能的其他来源。
Previous observations have shown that the striatum contains a population of neurones that display immunoreactivity for calretinin. In order to morphologically characterize these neurones, sections of the rat striatum were immunostained to reveal calretinin and examined at both light and electron microscopic levels. The striatum contained a small population of calretinin-immunoreactive neurones, which were of medium-size (9-17 mum) and possessed few aspiny, infrequently branching dendrites which tapered to become very thin processes in their most distal portions. Although the calretinin-immunoreactive neurones were homogeneously distributed in the frontal plane, there was a marked rostrocaudal gradient with a much greater density of cells in the rostral than in the caudal parts of the striatum. At the ultrastructural level, calretinin-immunoreactive neurones were seen to possess an indented nucleus and to receive synaptic input from at least three types of boutons. In addition to the calretinin-immunoreactive neurones, the striatum also contained axons and terminal boutons that displayed immunoreactivity for calretinin. At least two types of immunoreactive terminals were identified, those forming symmetrical synaptic specialisations and those forming asymmetrical synaptic specialisations. Approximately 50% formed asymmetrical contacts with spines and 30% formed symmetrical synaptic contact with dendritic shafts. In an attempt to further chemically characterize the calretinin-containing neurones, double pre-embedding immunocytochemistry for calretinin and parvalbumin or choline acetyltransferase was carried out and calretinin immunocytochemistry was combined with histochemistry for NADPH-diaphorase. Analysis of these double-stained sections revealed that the population of calretinin-immunoreactive neurones was distinct from the populations of neurones containing parvalbumin, choline acetyltransferase or NADPH-diaphorase. It is concluded that: (1) on the basis of distribution, morphology, chemistry, ultrastructure and afferent synaptic input, the calretinin-immunoreactive neurones are distinct from the major classes of neurones that have been previously recognised in the striatum; (2) calretinin-immunoreactive terminals are heterogeneous and are probably derived from local calretinin-containing neurones and possibly other sources.