Effect of Sulfadiazine on Abundance and Diversity of Denitrifying Bacteria by Determining nirK and nirS Genes in Two Arable Soils
Effect of Sulfadiazine on Abundance and Diversity of Denitrifying Bacteria by Determining nirK and nirS Genes in Two Arable Soils
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DOI：
10.1007/s00248-010-9691-9
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发表时间：
2010-06
影响因子：
3.6
通讯作者：
K. Kleineidam;Shilpi Sharma;Anja Kotzerke;H. Heuer;S. Thiele-Bruhn;K. Smalla;B. Wilke;M. Schloter
中科院分区：
文献类型：
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作者：
K. Kleineidam;Shilpi Sharma;Anja Kotzerke;H. Heuer;S. Thiele-Bruhn;K. Smalla;B. Wilke;M. Schloter
磺胺嘧啶（SDZ）是一种常用的抗生素在农业畜牧业。通过对药用动物的排泄物进行施肥，药物到达土壤并可能损害微生物进行的重要生化转化过程，例如，氮的周转。通过对两种农田土壤亚硝酸盐还原酶基因的定量分析，研究了猪粪和添加SDZ的猪粪对土壤中微生物的影响。添加有机肥主要导致两种土壤中nirK-携带的微生物的增加，而在SDZ-修正的处理中，主要是nirK-携带的微生物在生物可利用的SDZ下降后大量增加。然而，社区组成ofnirS亚硝酸盐还原剂的变性梯度凝胶电泳研究没有改变，尽管观察到的丰度的变化。
Sulfadiazine (SDZ) is an antibiotic frequently used in agricultural husbandry. Via manuring of excrements of medicated animals, the drug reaches the soil and might impair important biochemical transformation processes performed by microbes, e.g., the nitrogen turnover. We studied the effect of pig manure and SDZ-spiked pig manure on denitrifying bacteria by quantifyingnirKandnirSnitrite reductase genes in two arable soils. Addition of manure entailed mainly an increase ofnirK-harboring denitrifiers in both soils, whereas in the SDZ-amended treatments, primarily thenirSdenitrifiers increased in abundance after the bioavailable SDZ had declined. However, the community composition ofnirSnitrite reducers investigated by denaturing gradient gel electrophoresis did not change despite the observed alterations in abundance.