ANALYSES OF HOMOLOGOUS ROTAVIRUS INFECTION IN THE MOUSE MODEL
ANALYSES OF HOMOLOGOUS ROTAVIRUS INFECTION IN THE MOUSE MODEL
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DOI：
10.1006/viro.1995.1060
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发表时间：
1995-02-20
期刊：
影响因子：
3.7
通讯作者：
GREENBERG, HB
中科院分区：
文献类型：
--
作者：
BURNS, JW;KRISHNANEY, AA;GREENBERG, HB
A组轮状病毒在发病率、死亡率和经济损失方面是重要的人类和兽医病原体。尽管它很重要，但有效的疫苗仍然难以捉摸，至少部分原因是我们对轮状病毒免疫和保护的不完全了解。已经建立了大型和小型动物模型系统来解决这些问题。这些模型的一个显着缺点是缺乏充分表征的野生型同源病毒及其细胞培养适应的变体。我们使用每种毒株的野生型分离株和适应细胞培养的变体，在婴儿和成年小鼠模型中鉴定了四种鼠轮状病毒株：EC、EHP、EL 和 EW。就初次感染后病毒脱落的强度和持续时间而言，野生型鼠轮状病毒在婴儿和成年小鼠中似乎具有相同的传染性。在两个年龄组中都观察到感染向幼稚的笼友传播。原发感染后脱落的清除似乎与病毒特异性肠道 IgA 的发展相关。口腔感染后，婴儿和成年小鼠都会产生保护性免疫力，随后的野生型病毒攻击后没有脱落就证明了这一点。当对幼稚动物施用时，适应细胞培养的鼠轮状病毒似乎高度减毒，并且不会有效传播至非免疫笼中动物。这些野生型和细胞培养适应病毒制剂的可用性应该可以对轮状病毒感染和免疫进行更系统的评估。此外，可以使用几种完全毒力的同源病毒进行攻击，在小鼠模型中评估未来的疫苗策略。 (C) 1995 学术出版社
The group A rotaviruses are significant human and veterinary pathogens in terms of morbidity, mortality, and economic loss. Despite its importance, an effective vaccine remains elusive due at least in part to our incomplete understanding of rotavirus immunity and protection. Both large and small animal model systems have been established to address these issues. One significant drawback of these models is the lack of well-characterized wild-type homologous Viruses and their cell culture-adapted variants. We have characterized four strains of murine rotaviruses, EC, EHP, EL, and EW, in the infant and adult mouse model using wild-type isolates and cell culture-adapted variants of each strain. Wild-type murine rotaviruses appear to be equally infectious in infant and adult mice in terms of the intensity and duration of virus shedding following primary infection. Spread of infection to naive cagemates is seen in both age groups. Clearance of shedding following primary infection appears to correlate with the development of virus-specific intestinal IgA. Protective immunity is developed in both infant and adult mice following oral infection as demonstrated by a lack of shedding after subsequent wild-type virus challenge. Cell culture-adapted murine rotaviruses appear to be highly attenuated when administered to naive animals and do not spread efficiently to nonimmune cagemates. The availability of these wild-type and cell culture-adapted virus preparations should allow a more systematic evaluation of rotavirus infection and immunity. Furthermore, future vaccine strategies can be evaluated in the mouse model using several fully virulent homologous viruses for challenge. (C) 1995 Academic Press, Inc.