Detection of nandrolone, testosterone, and their esters in rat and human hair samples.
Detection of nandrolone, testosterone, and their esters in rat and human hair samples.
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检测大鼠和人头发样本中的诺龙、睾酮及其酯。
DOI：
10.1093/jat/23.6.416
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发表时间：
1999
影响因子：
2.5
通讯作者：
JosephJr,RE
中科院分区：
文献类型：
--
作者：
Höld,KM;Borges,CR;Wilkins,DG;Rollins,DE;JosephJr,RE
诺龙和睾酮是合成代谢雄激素类固醇，偶尔被运动员滥用。开发了一种灵敏、特异且可重复的气相色谱-质谱法，用于定量测定头发中的诺龙、睾酮及其酯。基于 20 mg 头发，该方法的定量限为：诺龙和睾酮为 50 pg/mg，醋酸睾酮为 100 pg/mg，癸酸诺龙为 200 pg/mg。诺龙-d3 和睾酮-d3 用作内标。该方法已应用于分析掺入大鼠和人类头发中的这些化合物。雄性 Long-Evans 大鼠在 14 天的时间段内每天一次腹膜内 (ip) 给予癸酸诺龙 60 mg/kg，持续 10 天。三只大鼠中的两只在第 21 天收集的色素毛发中含有诺龙，浓度分别为 63 皮克/毫克和 76 皮克/毫克。在相应的非色素头发中没有发现药物。诺龙检测呈阳性的大鼠毛发样本中还含有浓度分别为 0.9 ng/mg 和 1.2 ng/mg 的癸酸诺龙。在另一项实验中，大鼠每天一次腹腔注射醋酸睾酮 10 mg/kg，持续五天。在大鼠毛发样本中未检测到睾酮或醋酸睾酮。还从四名自我报告的类固醇使用者身上获取了头发样本。两名受试者的头发被确定为浓度为 54 皮克/毫克和 81 皮克/毫克的睾酮呈阳性。这些数据表明，全身给药后可以检测头发中的类固醇诺龙、睾酮和癸酸诺龙。
Nandrolone and testosterone are anabolic androgenic steroids occasionally abused by athletes. A sensitive, specific, and reproducible gas chromatography-mass spectrometry method for the quantitative determination of nandrolone, testosterone, and their esters in hair has been developed. The limits of quantitation of this method, based on 20 mg of hair, were 50 pg/mg for nandrolone and testosterone, 100 pg/mg for testosterone acetate, and 200 pg/mg for nandrolone-decanoate. Nandrolone-d3 and testosterone-d3 were used as internal standards. This method has been applied to the analysis of these compounds incorporated into rat and human hair. Male Long-Evans rats were given nandrolone decanoate 60 mg/kg intraperitoneally (ip) once daily for 10 days over a time period of 14 days. Two of the three rats contained nandrolone in the pigmented hair collected at day 21 at a concentration of 63 and 76 pg/mg, respectively. No drug was found in the corresponding nonpigmented hair. The rat hair samples that tested positive for nandrolone contained also nandrolone decanoate in concentrations of 0.9 and 1.2 ng/mg, respectively. In a separate experiment rats were given testosterone acetate 10 mg/kg ip once daily for five days. No testosterone or testosterone acetate was detected in the rat hair samples. Hair specimens were also obtained from four self-reported steroid users. The hair of two subjects were determined to be positive for testosterone in concentrations of 54 and 81 pg/mg. These data demonstrate that it is possible to detect the steroids nandrolone, testosterone, and nandrolone decanoate in hair after systemic administration.