Immune evaluation of biomaterials in TNF-α and IL-1β at mRNA level
Immune evaluation of biomaterials in TNF-α and IL-1β at mRNA level
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DOI：
10.1007/s10856-007-3014-9
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发表时间：
2007-11-01
影响因子：
3.7
通讯作者：
Zhang, Ping
中科院分区：
文献类型：
--
作者：
Ding, Tingting;Sun, Jiao;Zhang, Ping
目的探讨生物材料的生物相容性，改进现有的免疫学评价方法。方法以肿瘤坏死因子α（TNF-α）和白细胞介素1 β（IL 1-β）为指标，从mRNA水平检测生物材料的生物相容性。方法用不同材料刺激大鼠腹腔巨噬细胞，用逆转录聚合酶链反应（RT-PCR）检测TNF-α和IL 1 β的表达，并进行比较。结果PTFE刺激后巨噬细胞产生的TNF-α和IL 1 β明显高于对照组（P < 0.05）。结论PTFE刺激后巨噬细胞产生的TNF-α和IL 1 β明显高于对照组（P < 0.05）。B比未刺激的巨噬细胞（p < 0.001）。PLGA、NPG、β-TCP和CPC组也诱导了中等水平的TNF-α和IL 1-B表达（p < 0.01）。结论TNF-α和IL 1-β是免疫刺激的敏感指标，有助于监测不同生物材料诱导的细胞活化水平。我们的研究结果表明，β-TCP和CPC具有良好的生物相容性，CPC是所有测试的生物材料中生物相容性最好的。而PTFE和NPG植入体内后仍会产生促炎细胞因子，有一定的副作用。
Objective To examine biomaterial biocompatibility and improve current methods of immunological evaluation TNF-alpha and IL1-beta were used as indicators at mRNA levels.Methods Rat peritoneal macrophages were stimulated with different biomaterials and expression of TNF-alpha and IL1-beta was measured by RT-PCR and compared within different groups.Results Macrophages that were stimulated with PTFE produced significantly more TNF-a and IL1-b than unstimulated macrophages (p < 0.001). The PLGA, NPG, beta-TCP, and CPC groups also induced moderate TNF-alpha and IL1-b expression (p < 0.01).Conclusions TNF-alpha and IL1-beta are sensitive indicators of immune stimulation that can help to monitor the levels of cellular activation induced by different biomaterials. Our findings showed that beta-TCP and CPC had a good biocompability, and CPC was the most biocompatible of all the biomaterials tested. While PTFE and NPG still had side effect because of producing pro-inflammatory cytokines in vitro once implanted.