FLP RECOMBINASE IS AN ENZYME
FLP RECOMBINASE IS AN ENZYME
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DOI：
10.1073/pnas.85.13.4628
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发表时间：
1988-07-01
影响因子：
11.1
通讯作者：
COX, MM
中科院分区：
文献类型：
--
作者：
GATES, CA;COX, MM
酵母2-.mu.m质粒的FLP蛋白催化分子间位点特异性重组，周转数为.apprxeq。对于松弛的 DNA 底物，0.12 min-1（每个 FLP 单体）。在增强其稳定性的条件下，蛋白质可以以催化量而不是化学计量量使用。即使当蛋白质相对于可用重组位点过量存在时，反应速率也表现出对 FLP 蛋白质浓度的强烈依赖性。
The FLP protein of the yeast 2-.mu.m plasmid catalyzes intermolecular site-specific recombination with a turnover number of .apprxeq. 0.12 min-1 (per FLP monomer) for relaxed DNA substrates. Under conditions that enhance its stability, the protein can be used in catalylic rather than stoichiometric amounts. The reaction rate rate exhibits a strong dependence on FLP protein concentration even when the protein is present in excess relative to available recombination sites.