A mouse strain defective for alphabeta versus gammadelta T cell lineage commitment.
A mouse strain defective for alphabeta versus gammadelta T cell lineage commitment.
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Alphabeta 与 gammadelta T 细胞谱系定型存在缺陷的小鼠品系。
DOI：
10.1093/intimm/dxf067
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发表时间：
2002
影响因子：
4.4
通讯作者：
Hedrick,StephenM
中科院分区：
文献类型：
--
作者：
Mertsching,Elisabeth;Wurster,AndreaL;Katayama,Carol;Esko,Jeffrey;Ramsdell,Fred;Marth,JameyD;Hedrick,StephenM
由于转基因插入和染色体缺失，一种突变小鼠品系在产生αβ和γδ T细胞平衡的谱系承诺步骤中存在缺陷。小鼠产生的αβ CD4 T细胞数量减少，几乎没有αβ CD8 T细胞。在胸腺的CD4和CD8群体中存在表达γδ TCR的异常亚群。这些表达γδ TCR的细胞分布在外周淋巴器官中，淋巴结和脾脏中高达75%的CD8 T细胞表达γδ TCR。进一步的分析表明，阻止双TCR表达的调控已经受损。插入和删除位点定位在10 26 cM染色体上。我们分析了整个基因座，并利用基因阵列技术分析了早期胸腺细胞的基因表达变化。对该突变株的分析表明，αβ与γδ谱系的决定可以被严重失调，而不依赖于成功的基因重排。
As a result of a transgene insertion and chromosomal deletion, a mutant mouse strain has been found that is defective in the lineage commitment step that produces a balance of αβ and γδ T cells. The mice produce a reduced population of αβ CD4 T cells and almost no αβ CD8 T cells. Within the CD4 and CD8 populations in the thymus there exist abnormal subsets that express the γδ TCR. These γδ TCR‐expressing cells populate the peripheral lymphoid organs such that up to 75% of the CD8 T cells in the lymph nodes and spleen express a γδ TCR. Further analyses indicate that the regulation that prevents dual TCR expression has been impaired. The locus of insertion and deletion was mapped to chromosome 10 26 cM. We have analyzed the entire locus and, in addition, the gene expression changes in early thymocytes were analyzed by gene array technology. The analysis of this mutant strain indicates that the αβ versus γδ lineage decision can be profoundly disregulated independently of successful gene rearrangements.