PHOSPHOLIPASE-C ASSAY USING PARA-NITROPHENYLPHOSPHORYLCHOLINE TOGETHER WITH SORBITOL AND ITS APPLICATION TO STUDYING METAL AND DETERGENT REQUIREMENT OF ENZYME
PHOSPHOLIPASE-C ASSAY USING PARA-NITROPHENYLPHOSPHORYLCHOLINE TOGETHER WITH SORBITOL AND ITS APPLICATION TO STUDYING METAL AND DETERGENT REQUIREMENT OF ENZYME
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DOI：
10.1016/0003-2697(76)90078-6
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发表时间：
1976-01-01
影响因子：
2.9
通讯作者：
MATSUDA, M
中科院分区：
文献类型：
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作者：
KURIOKA, S;MATSUDA, M
在含有山梨醇的培养基中，磷脂酶C [EC 3.1.4.3]对对硝基苯基磷酰胆碱的水解速率大大加快。甘油在增加水解速率方面也是有效的，但不像山梨糖醇那样增加水解速率。本文提出了一种简便的磷脂酶C分光光度测定法，以对硝基苯磷酸胆碱和山梨醇（60%，wt/wt）为显色剂。该方法对研究磷脂酶C对金属离子和去污剂的需要量具有重要意义。使用这种方法获得的结果表明，产气荚膜梭菌的磷脂酶C是一种金属酶，可能需要Zn 2+，没有类型的洗涤剂有任何激活作用的酶本身。
The hydrolytic rate of p-nitrophenylphosphorylcholine by phospholipase C [EC 3.1.4.3] is highly accelerated in a medium containing sorbitol. Glycerol is also effective in increasing the hydrolytic rate but does not increase it as much as sorbitol. A convenient spectrophotometric assay method for phospholipase C using p-nitrophenylphosphorylcholine together with sorbitol (60%, wt/wt) was proposed. This method was very useful for studying the metal and detergent requirement of phospholipase C. The results obtained using this method suggest that phospholipase C of Clostridium perfringens is a metalloenzyme possibly requiring Zn2+ and that no type of detergent has any activating effect on the enzyme itself.