Antioxidant activity of the flaxseed lignan secoisolariciresinol diglycoside and its mammalian lignan metabolites enterodiol and enterolactone
Antioxidant activity of the flaxseed lignan secoisolariciresinol diglycoside and its mammalian lignan metabolites enterodiol and enterolactone
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DOI：
10.1023/a:1007022329660
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发表时间：
1999-12-01
影响因子：
4.3
通讯作者：
Thompson, LU
中科院分区：
文献类型：
--
作者：
Kitts, DD;Yuan, YV;Thompson, LU
亚麻籽木脂素开环异落叶松树脂酚二糖苷（SDG）及其哺乳动物木脂素代谢产物肠二醇（艾德）和肠内酯（EL）的抗氧化活性，在脂质和水在体外模型系统进行了评价。在40 ℃下孵育24-48小时后，所有三种木脂素在10和100 μ M下均显著抑制亚油酸过氧化（p小于或等于0.05）。在脱氧核糖测定中，其评价非位点特异性和位点特异性芬顿反应诱导的。OH清除活性，SDG在10和100 μ M下表现出与艾德和EL相比最弱的活性;在两种测定中，在100 μ M下观察到艾德和EL的OH清除。芬顿试剂与SDG、艾德或EL共同作用于pBR 322质粒DNA时，对DNA断裂的抑制作用呈浓度依赖性。木脂素的最大非位点特异性活性是在100 μ M，因此，证实了脱氧核糖试验的结果。相比之下，SDG和EL在位点特异性测定中的保护作用丧失，而艾德的保护作用极小。因此，结果表明三种木脂素之间的结构-活性差异，具体的抗氧化功效。所有三个木脂素没有表现出还原活性相比，抗坏血酸，因此，不具有间接的促氧化活性相关的过渡金属氧化还原状态的潜在变化。SDG和特别是哺乳动物木脂素艾德和EL在脂质和水性体外模型系统中以相对低的浓度（即100 μ M）（可能在体内实现）充当抗氧化剂的功效是亚麻籽木脂素SDG及其哺乳动物代谢物艾德和EL的潜在抗癌机制的证据。
The antioxidant activities of the flaxseed lignan secoisolariciresinol diglycoside (SDG) and its mammalian lignan metabolites, enterodiol (ED) and enterolactone (EL), were evaluated in both lipid and aqueous in vitro model systems. All three lignans significantly (p less than or equal to 0.05) inhibited the linoleic acid peroxidation at both 10 and 100 mu M over a 24-48 h of incubation at 40 degrees C. In a deoxyribose assay, which evaluates the non site-specific and site-specific Fenton reactant-induced . OH scavenging activity, SDG demonstrated the weakest activity compared to ED and EL at both 10 and 100 mu M; the greatest . OH scavenging for ED and EL was observed at 100 mu M in both assays. The incubation of pBR322 plasmid DNA with Fenton reagents together with SDG, ED or EL showed that the inhibition of DNA scissions was concentration dependent. The greatest non site-specific activity of lignans was at 100 mu M, thus, confirming the results of the deoxyribose test. In contrast, the protective effect of SDG and EL in the site-specific assay was lost and that of ED was minimal. Therefore, the results indicate a structure-activity difference among the three lignans with respect to specific antioxidant efficacy. All three lignans did not exhibit reducing activity compared to ascorbic acid, therefore, did not possess indirect prooxidant activity related to potential changes in redox state of transition metals. The efficacy of SDG and particularly the mammalian lignans ED and EL to act as antioxidants in lipid and aqueous in vitro model systems, at relatively low concentrations (i.e. 100 mu M), potentially achievable in vivo, is an evidence of a potential anticarcinogenic mechanism of flaxseed lignan SDG and its mammalian metabolites ED and EL.