Conjugation and fluorescence quenching between bovine serum albumin and L-cysteine capped CdSe/CdS quantum dots.
Conjugation and fluorescence quenching between bovine serum albumin and L-cysteine capped CdSe/CdS quantum dots.
复制标题
牛血清白蛋白和 L-半胱氨酸封端的 CdSe/CdS 量子点之间的缀合和荧光猝灭。
DOI：
10.2174/092986611794653905
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发表时间：
2011
影响因子：
1.6
通讯作者：
Xi Li
中科院分区：
文献类型：
--
作者：
Qisui Wang;Fangyun Ye;P. Liu;Xinmin Min;Xi Li
以L-半胱氨酸为封端剂，在水溶液中合成了水溶性、生物相容性、荧光性能优异的CdSe/CdS量子点。采用荧光光谱、吸收光谱和透射电镜对产品进行了表征。通过吸附实验和荧光滴定实验研究了量子点与牛血清白蛋白（BSA）的相互作用。随着量子点的加入，BSA的荧光强度显着淬灭，这可以解释在自然界中的静态机制。当BSA加入到量子点溶液中时，量子点的荧光强度被微弱地猝灭。荧光成像表明，量子点可以被设计成一种探针来标记土方大肠杆菌（E。coli）细胞。这些结果表明，CdSe/CdS/L-半胱氨酸量子点可作为生物分子和细菌细胞标记的探针。
Water-soluble, biological-compatible, and excellent fluorescent CdSe/CdS quantum dots (QDs) with L-cysteine as capping agent were synthesized in aqueous medium. Fluorescence (FL) spectra, absorption spectra, and transmission electron microscopy (TEM) were employed to investigate the quality of the products. The interactions between QDs and bovine serum albumin (BSA) were studied by absorption and FL titration experiments. With addition of QDs, the FL intensity of BSA was significantly quenched which can be explained by static mechanism in nature. When BSA was added to the solution of QDs, FL intensity of QDs was faintly quenched. Fluorescent imaging suggests that QDs can be designed as a probe to label the Escherchia coli (E. coli) cells. These results indicate CdSe/CdS/L-cysteine QDs can be used as a probe for labeling biological molecule and bacteria cells.