Validated LC-MS (ESI) assay for the simultaneous determination of amitriptyline and its metabolite nortriptyline in rat plasma: application to a pharmacokinetic comparison.
Validated LC-MS (ESI) assay for the simultaneous determination of amitriptyline and its metabolite nortriptyline in rat plasma: application to a pharmacokinetic comparison.
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DOI：
10.1016/j.jpba.2010.04.031
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发表时间：
2010-11
影响因子：
3.4
通讯作者：
Yuan Shen;R. Zhu;Huande Li;Yan-Wen Liu;P. Xu
中科院分区：
文献类型：
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作者：
Yuan Shen;R. Zhu;Huande Li;Yan-Wen Liu;P. Xu
建立了同时测定大鼠血浆中阿米替林和去甲替林浓度的高效液相色谱-电喷雾质谱（HPLC-MS/ESI）方法。样品经0.5mol/l NaOH碱化后，用甲基叔丁基醚进行液-液萃取。色谱柱为XB-C4（4.6mm× 250 mm，5μm，Welch Materials），移动的相为10 mM醋酸铵（0.6‰甲酸）-乙腈（60：40，v/v），流速为1.0ml/min，阿米替林和去甲替林的线性范围分别为10- 3200 ng/ml和10- 1000 ng/ml。本方法已成功地应用于大鼠静脉注射盐酸阿米替林后的药代动力学研究。
A rapid, sensitive and specific method based on high performance liquid chromatography with electrospray ionization mass spectrometry (HPLC–MS/ESI) has been developed for the simultaneous determination of amitriptyline and nortriptyline in rat plasma. Sample preparation involved liquid–liquid extraction with methyl t-butyl ether after alkalified with 0.5mol/l NaOH. Chromatographic separation was performed on a XB-C4 column (4.6mm×250mm, 5μm, Welch Materials) with a mobile phase consisting of 10mM ammonium acetate (0.6‰ formic acid)–acetonitrile (60:40, v/v) at a flow rate of 1.0ml/min. Calibration curves were linear within the ranges of 10–3200ng/ml for amitriptyline and 10–1000ng/ml for nortriptyline. This method was successfully applied to the pharmacokinetic study in rats after intravenous injection of amitriptyline hydrochloride.