Fertilization in Trypsin–Treated Oocytes
Fertilization in Trypsin–Treated Oocytes
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胰蛋白酶处理的卵母细胞的受精
DOI：
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发表时间：
1988
影响因子：
5.2
通讯作者：
K. V. Jackson
中科院分区：
文献类型：
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作者：
A. Kiessling;D. Loutradis;P. Mcshane;K. V. Jackson
卵母细胞在体外受精失败可能是由于几种原因，包括配子相互作用缺陷或卵母细胞细胞质不能形成雄性和雌性原核。在精液分析结果在正常范围内，卵母细胞看起来成熟健康的情况下，情况尤其令人困惑。为了区分配子相互作用缺陷和卵母细胞细胞质功能障碍，去除阻碍精子穿透卵膜的透明带将是有益的。随后出现的原核表明卵母细胞细胞质功能充足，暗示受精失败是配子相互作用的缺陷。轻度胰蛋白酶化治疗削弱带屏障是一种可能的方法来理解这个问题，因为这种治疗通常应用于培养细胞，没有明显的膜损伤证据。我们在此报告使用轻度胰蛋白酶化来改善三个有受精失败史的患者的卵母细胞受精。
Failure of oocytes to fertilize in vitro may be due to several causes, including defective gamete interaction or inability of oocyte cytoplasm to form male and female pronuclei. The situation is particularly confusing in those cases in which results of the semen analyses are within normal limits, and the oocytes appear mature and healthy. To distinguish between defective gamete interaction and oocyte cytoplasmic dysfunction, it would be useful to remove the zona pellucida barrier to sperm penetration of the oolemma. The subsequent appearance of pronuclei would indicate adequate oocyte cytoplasmic functions and imply that fertilization failure was a defect of gamete interaction. Mild trypsinization treatment to weaken the zona barrier is a possible approach to understanding the problem since such treatment is routinely applied to cells in culture without evidence of significant membrane damage. We report here the use of mild trypsinization to improve fertilization of oocytes from three patients with a history of fertilization failure.
在不含蛋白质的 Hams F-10 培养基中，在扩展的 pH 范围内改善了原核小鼠胚胎的发育。
影响因子：
6.7
作者：
John,DP;Kiessling,AA
通讯作者：
Kiessling,AA