Cell wall alterations in loblolly pine wood decayed by the white-rot fungus, Ceriporiopsis subvermispora
Cell wall alterations in loblolly pine wood decayed by the white-rot fungus, Ceriporiopsis subvermispora
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DOI：
10.1016/s0168-1656(97)01674-x
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发表时间：
1997-03-14
影响因子：
4.1
通讯作者：
Akin, DE
中科院分区：
文献类型：
--
作者：
Blanchette, RA;Krueger, EW;Akin, DE
采用超微结构、免疫细胞化学和紫外吸收光谱技术研究了木本植物枯败过程中细胞壁发生的渐进性变化。孵育仅2周后，醋酸铀酰染色显示，在菌丝附近和细胞壁外周周围的次级壁有弥散的电子密集区。随着真菌培养时间的延长，细胞壁染色程度增加。未改变的细胞壁未见染色。不同分子量的蛋白质（胰岛素，5730 Da；肌红蛋白，17 600 Da；卵清蛋白，44 287 Da）浸润到声音和腐烂的木材中，然后进行免疫金标记和透射电镜。在腐烂2周后，胰岛素很容易渗透到木细胞次生壁的最外层。第一次观察到肌红蛋白在降解4周时穿透细胞壁，而卵清蛋白在8周后被发现，在腐烂的晚期，细胞壁明显破坏。所使用的蛋白质都没有定位于未经处理的对照木材样品的细胞壁内。紫外显微分光光度法显示，在整个衰变研究期间，在不同的采样时间，次级壁和中间薄片在240和280 nm处的吸光度逐渐丧失。(C) 1997爱思唯尔科学有限公司
Ultrastructural, immunocytochemical and UV absorption spectroscopy techniques were used to elucidate the progressive changes that occur within woody cell walls during decay by Ceriporiopsis subvermispora. After only 2 weeks of incubation, uranyl acetate staining revealed a diffuse electron dense zone in the secondary wall near hyphae and around the outer circumference of the wall. The extent of cell wall staining increased with longer fungal incubation. No staining occurred in sound unaltered cell walls. Proteins of varying molecular weights (insulin, 5730 Da; myoglobin, 17 600 Da; ovalbumin, 44 287 Da) were infiltrated into sound and decayed wood followed by immunogold labelling and transmission electron microscopy. Insulin readily penetrated into the outer most regions of secondary walls of wood cells after 2 weeks of decay. Myoglobin was first observed to penetrate cell walls at 4 weeks of degradation and ovalbumin was found after 8 weeks in wood with advanced stages of decay where extensive cell wall disruption was evident. None of the proteins used were localized within cell walls of untreated, control wood samples. UV microspectrophotometry demonstrated a progressive loss of absorbance at 240 and 280 nm within the secondary walls and middle lamellae at various sampling times throughout the duration of the decay study. (C) 1997 Elsevier Science B.V.