Localization and Expression of MreB in Vibrio parahaemolyticus under Different Stresses
Localization and Expression of MreB in Vibrio parahaemolyticus under Different Stresses
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DOI：
10.1128/aem.01020-08
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Wong, Hin-chung
中科院分区：
文献类型：
--
作者：
Chiu, Shen-Wen;Chen, Shau-Yan;Wong, Hin-chung
MreB是真核生物肌动蛋白的同源物，在原核生物通过改变其空间组织（包括形态、亚细胞结构和大分子定位）科普压力时可能发挥至关重要的作用。本研究探讨了副溶血性弧菌中MreB在不同应激条件下的行为。在部分二倍体菌株SC 9中使用黄色荧光蛋白-MreB缀合物探测MreB的行为。在正常生长条件下，MreB在指数期细胞中形成螺旋丝。饥饿或静止期细胞的形状由杆状变为小球状。细胞通过“溶胀-衰退”过程分化为具有小球形细胞的活的但不可培养（VBNC）状态。在所有情况下，观察到MreB细胞骨架的剧烈重塑。在这些压力下，细菌中的MreB螺旋通常被松开并断裂成短细丝、弧形和斑点。解体的MreB表现出强烈的倾向，附着在细胞质膜上。mreB的表达在细菌的稳定期和饥饿下普遍下降，但在冷休克和VBNC状态分化的初始阶段上调，随后下降。我们的研究结果表明，MreB的行为在副溶血性弧菌的形态变化的内在或外在的压力，并可能有重要的意义，研究细胞的应激反应和衰老。
MreB, the homolog of eukaryotic actin, may play a vital role when prokaryotes cope with stress by altering their spatial organization, including their morphology, subcellular architecture, and localization of macromolecules. This study investigates the behavior of MreB in Vibrio parahaemolyticus under various stresses. The behavior of MreB was probed using a yellow fluorescent protein-MreB conjugate in merodiploid strain SC9. Under normal growth conditions, MreB formed helical filaments in exponential-phase cells. The shape of starved or stationary-phase cells changed from rods to small spheroids. The cells differentiated into the viable but nonculturable (VBNC) state with small spherical cells via a "swelling-waning" process. In all cases, drastic remodeling of the MreB cytoskeleton was observed. MreB helices typically were loosened and fragmented into short filaments, arcs, and spots in bacteria under these stresses. The disintegrated MreB exhibited a strong tendency to attach to the cytoplasmic membrane. The expression of mreB generally declined in bacteria in the stationary phase and under starvation but was upregulated during the initial periods of cold shock and VBNC state differentiation and decreased afterwards. Our findings demonstrated the behavior of MreB in the morphological changes of V. parahaemolyticus under intrinsic or extrinsic stresses and may have important implications for studying the cellular stress response and aging.