Large-scale comparison of Roche Cobas AmpliPrep/Cobas TaqMan and Abbott Real Time HIV assays
Large-scale comparison of Roche Cobas AmpliPrep/Cobas TaqMan and Abbott Real Time HIV assays
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DOI：
10.1016/j.jviromet.2012.05.007
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发表时间：
2012-09-01
影响因子：
3.1
通讯作者：
Hillyard, David R.
中科院分区：
文献类型：
--
作者：
Pyne, Michael T.;Wilson, Andrew;Hillyard, David R.
据报道，与其他检测方法相比，罗氏 Cobas AmpliPrep/Cobas TaqMan HIV 检测（第 1 版）的 HIV RNA 定量显着不足。然而，这些研究通常涉及来自选定患者群体的有限数量的样本或对 TaqMan 检测中无法检测到的样本进行分析。在 Roche TaqMan 和 Abbott RealTime 检测中对从美国各地提交的用于 HIV RNA 定量的随机血浆样本 (n = 1263) 进行了比较。 24 个样本 (1.9%) 存在差异，两种检测之间的最大差异为 1.9 log 拷贝/mL。这些数据表明，这两种检测可能容易出现定量不足的情况，但在来自美国各地的这一大样本群中，发生率较低。 (C) 2012 Elsevier B.V. 保留所有权利。
Significant underquantitation of HIV RNA has been reported with the Roche Cobas AmpliPrep/Cobas TaqMan HIV Test (Version 1) compared to other assays. However, these studies have generally involved limited numbers of samples from select patient populations or analysis of samples that were undetectable in the TaqMan assay. Random plasma samples submitted from throughout the United States for HIV RNA quantitation (n = 1263) were compared in the Roche TaqMan and Abbott RealTime assays. Twenty-four samples (1.9%) were discrepant, with a maximum difference between the two assays of 1.9 log copies/mL These data indicate that both tests may be susceptible to underquantitation, but the incidence is low in this large cohort of samples from across the United States. (C) 2012 Elsevier B.V. All rights reserved.