Expression of zein in long term endosperm cultures of maize.
Expression of zein in long term endosperm cultures of maize.
复制标题
玉米醇溶蛋白在玉米胚乳长期培养物中的表达。
DOI：
10.1104/pp.73.4.915
复制
发表时间：
1983
期刊：
影响因子：
7.4
通讯作者：
C. Dierks
中科院分区：
文献类型：
--
作者：
K. Shimamoto;M. Ackermann;C. Dierks
自交系A636玉米胚乳授粉后10天建立连续培养体系。21个月后用水乙醇提取。用聚丙烯酰胺-十二烷基硫酸钠凝胶电泳法对溶解蛋白进行分析。玉米的两条蛋白质带与玉米的主要贮藏蛋白玉米醇溶蛋白发生共迁移。对凝胶进行免疫印迹，然后将固定的蛋白与抗玉米醇溶蛋白抗体孵育，提供了多肽实际上是玉米醇溶蛋白的证据。电子显微镜研究表明，培养物中含有与发育中的胚乳相同的蛋白质小体。蛋白小体可以从培养物中分离出来，并被证明含有玉米醇溶蛋白。我们得出的结论是，这里描述的长期培养会合成玉米醇溶蛋白，并以发育中的胚乳中发现的那种蛋白质小体的形式沉积。因此，某些胚乳特征和组织特异性蛋白的产生在长期培养中保持不变。
Continuous cultures, established 10 days after pollination from endosperms of inbred A636 Zea mays (L.) were extracted 21 months later with aqueous ethanol. The solubilized proteins were analyzed by poly-acrylamide-sodium dodecyl sulfate gel electrophoresis. Two protein bands co-migrated with zein, the major storage protein of maize. Immunoblotting of the gel followed by incubation of the immobilized proteins with anti-zein IgG provided evidence that the polypeptides were in fact zein. Electron microscopic studies showed that the cultures contained cells with protein bodies as found in developing endosperms. The protein bodies could be isolated from the cultures and were shown to contain zein. We conclude that the long term cultures described here synthesize zein and deposit it in the form of protein bodies of the type found in developing endosperms. Thus, certain endosperm characteristics and the production of tissue-specific proteins are retained in prolonged culture.