Fast fluorescence techniques for crystallography beamlines.
Fast fluorescence techniques for crystallography beamlines.
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用于晶体学光束线的快速荧光技术。
DOI：
10.1107/s0021889811016748
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发表时间：
2011
影响因子：
6.1
通讯作者：
Fischetti,RobertF
中科院分区：
文献类型：
--
作者：
Stepanov,Sergey;Hilgart,Mark;Yoder,DerekW;Makarov,Oleg;Becker,Michael;Sanishvili,Ruslan;Ogata,CraigM;Venugopalan,Nagarajan;Aragão,David;Caffrey,Martin;Smith,JanetL;Fischetti,RobertF
This paper reports on several developments of X-ray fluorescence techniques for macromolecular crystallography recently implemented at the National Institute of General Medical Sciences and National Cancer Institute beamlines at the Advanced Photon Source. These include (i) three-band on-the-fly energy scanning around absorption edges with adaptive positioning of the fine-step band calculated from a coarse pass; (ii) on-the-fly X-ray fluorescence rastering over rectangular domains for locating small and invisible crystals with a shuttle-scanning option for increased speed; (iii) fluorescence rastering over user-specified multi-segmented polygons; and (iv) automatic signal optimization for reduced radiation damage of samples.
