Targeted transfection of stem cells with sub-20 femtosecond laser pulses
Targeted transfection of stem cells with sub-20 femtosecond laser pulses
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DOI：
10.1364/oe.16.009357
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发表时间：
2008-06-23
期刊：
影响因子：
3.8
通讯作者：
Tempea, Gabriel
中科院分区：
文献类型：
--
作者：
Uchugonova, Aisada;Koenig, Karsten;Tempea, Gabriel
多光子显微镜已经成为基于多光子电离和等离子体形成的生物样品非接触式亚波长三维纳米加工的重要工具。需要超短激光脉冲，然而，色散效应限制了在焦平面可实现的最短脉冲持续时间。我们报道了一台紧凑的非线性激光扫描显微镜，它具有低于20飞秒的75 MHz近红外激光脉冲，用于人类干细胞的纳米手术和双光子高分辨率成像。对细胞膜进行单点照射，以诱导瞬时纳米孔传递细胞外绿色荧光蛋白质粒。在这些初步研究中，平均功率小于7 mW(<；93pJ)和低毫秒暴露时间被发现足以转染人胰腺和唾液腺干细胞。超小型亚20飞秒激光显微镜可能成为纳米生物技术和纳米医学的光学工具，包括光学干细胞操作。(C)2008年美国光学学会。
Multiphoton microscopes have become important tools for non-contact sub-wavelength three-dimensional nanoprocessing of living biological specimens based on multiphoton ionization and plasma formation. Ultrashort laser pulses are required, however, dispersive effects limit the shortest pulse duration achievable at the focal plane. We report on a compact nonlinear laser scanning microscope with sub-20 femtosecond 75 MHz near infrared laser pulses for nanosurgery of human stem cells and two-photon high-resolution imaging. Single point illumination of the cell membrane was performed to induce a transient nanopore for the delivery of extracellular green fluorescent protein plasmids. Mean powers of less than 7 mW (< 93 pJ) and low millisecond exposure times were found to be sufficient to transfect human pancreatic and salivary gland stem cells in these preliminary studies. Ultracompact sub-20 femtosecond laser microscopes may become optical tools for nanobiotechnology and nanomedicine including optical stem cell manipulation. (C) 2008 Optical Society of America.