Dual-stimuli sensitive nanogels fabricated by self-association of thiolated hydroxypropyl cellulose
Dual-stimuli sensitive nanogels fabricated by self-association of thiolated hydroxypropyl cellulose
复制标题
DOI：
10.1039/c0py00348d
复制
发表时间：
2011-02
影响因子：
4.6
通讯作者：
Junjun Tan;Hongliang Kang;Ruigang Liu;Deqian Wang;Xin Jin;Qinmei Li;Yong Huang
中科院分区：
文献类型：
--
作者：
Junjun Tan;Hongliang Kang;Ruigang Liu;Deqian Wang;Xin Jin;Qinmei Li;Yong Huang
通过将半胱胺与羟丙基纤维素(HPC)偶联，合成了一种新型的纤维素衍生物，其硫醇基度可通过反应物的投料比来控制。硫代化的HPC(HPC-SH)保持了HPC的热敏性，HPC链上的硫醇基团可以被氧化成二硫键。对MG-63细胞的细胞毒性实验证明，HPC-SH对MG-63细胞无害。通过HPC-HS在45°C溶液中的自缔合可以制备纳米凝胶，然后将硫醇基团氧化成二硫键以稳定缔合结构。纳米凝胶的交联度可以通过硫代化HPC中的-SH取代度来控制。纳米凝胶的流体动力学半径可以通过调节自缔合过程中的交联度和初始硫代化HPC溶液的浓度来调节。纳米凝胶的流体力学半径可以随温度变化，加入还原剂二硫苏糖醇(DTT)可以发生解离过程。双刺激敏感纳米凝胶在药物控制释放、转移开关器件和传感器等方面具有潜在的应用前景。
A new type of cellulose derivative was synthesized by means of conjugating cysteamine to hydroxypropyl cellulose (HPC) and the degree of thiol groups could be controlled by the feed ratio of the reactants. The thiolated HPC (HPC–SH) maintains the thermosensitivity of HPC and the thiol groups on the HPC chain can be oxidized to disulfide bonds. Cytotoxity tests performed on MG-63 cells proved that HPC–SH is not harmful to the cells. Nanogels can be fabricated by the self-association of HPC–HS in the solution at 45 °C and then oxidation of thiol groups to disulfide bonds occurs to stabilize the associated structure. The crosslinking degree of the nanogels could be controlled by the substitution degree of thiol groups (–SH) in the thiolated HPC. The hydrodynamic radius of the nanogels can be tuned by adjusting the degree of crosslinking and the concentration of the initial thiolated HPC solution in the self-association process. The hydrodynamic radius of the nanogels can be changed with the temperature and the dissociation process can happen by adding the reducing agent dithiothreitol (DTT). The dual-stimuli sensitive nanogels may have potential applications in controlled drug release, transfer switch device and sensors.