New assay to detect low-affinity interactions and characterization of leukocyte receptors for collagen including leukocyte-associated Ig-like receptor-1 (LAIR-1).
New assay to detect low-affinity interactions and characterization of leukocyte receptors for collagen including leukocyte-associated Ig-like receptor-1 (LAIR-1).
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DOI：
10.1002/eji.200839188
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发表时间：
2009-04
影响因子：
5.4
通讯作者：
Barclay, A. Neil
中科院分区：
文献类型：
--
作者：
Jiang, Lei;Barclay, A. Neil
关键词：
白细胞活性受细胞表面膜受体和配体之间的许多相互作用控制。这些相互作用具有低亲和力，使得检测困难。我们开发了一种灵敏的检测方法，可以在蛋白质水平上容易地检测极弱的相互作用，例如CD 200和活化受体CD 200 RLa（Kd>500μM）之间的相互作用。我们使用新技术来筛选胶原抑制受体的相互作用。我们证实人和小鼠白细胞相关免疫球蛋白样受体（LAIR-1）以及相关抑制性白细胞免疫球蛋白样受体亚家族B成员4（Lilrb 4，CD 85 K，Gp 49 B）特异性结合胶原蛋白，而其他细胞表面蛋白不结合。然后确定相互作用的单体亲和力，以允许与其他白细胞相互作用进行比较，并指示这种相互作用可能导致抑制信号的条件。
Leukocyte activity is controlled by numerous interactions between membrane receptors and ligands on the cell surface. These interactions are of low affinity making detection difficult. We developed a sensitive assay that could readily detect extremely weak interactions such as that between CD200 and the activating receptor CD200RLa (Kd>500μM) at the protein level. We used the new technology to screen for interaction of inhibitory receptors for collagens. We confirmed that both human and mouse leukocyte-associated immunoglobulin-like receptor (LAIR-1) and in addition the related inhibitory leukocyte immunoglobulin-like receptor subfamily B member 4 (Lilrb4, CD85K, Gp49B), bound collagen specifically whereas other cell surface proteins gave no binding. The monomeric affinities of the interactions were then determined to allow comparison with other leukocyte interactions and indicate conditions when this interaction might lead to inhibitory signals.
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影响因子：
--
作者：
Letarte, Michelle;Voulgaraki, Despina;Barclay, A. Neil
通讯作者：
Barclay, A. Neil
影响因子：
4.8
作者：
Miura, Y;Takahashi, T;Moroi, M
通讯作者：
Moroi, M
影响因子：
3.7
作者：
PFAFF, M;AUMAILLEY, M;TIMPL, R
通讯作者：
TIMPL, R
DOI：
10.1091/mbc.2.12.1035
发表时间：
1991-12-01
期刊：
CELL REGULATION
影响因子：
--
作者：
AGREZ, MV;BATES, RC;BURNS, GF
通讯作者：
BURNS, GF
影响因子：
20.3
作者：
Sarratt, KL;Chen, H;Kahn, ML
通讯作者：
Kahn, ML