DIFFERENTIAL REGULATION OF 2 DISTINCT FAMILIES OF GLUCOSE TRANSPORTER GENES IN TRYPANOSOMA-BRUCEI
DIFFERENTIAL REGULATION OF 2 DISTINCT FAMILIES OF GLUCOSE TRANSPORTER GENES IN TRYPANOSOMA-BRUCEI
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DOI：
10.1128/mcb.13.2.1146
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发表时间：
1993-02-01
影响因子：
5.3
通讯作者：
BALTZ, T
中科院分区：
文献类型：
--
作者：
BRINGAUD, F;BALTZ, T
一个串联排列的多基因家族编码推定的己糖转运蛋白在布氏锥虫的特点。它由两个80%同源的基因组组成，称为THT 1（6个拷贝）和THT 2（5个拷贝）。当非洲爪蟾卵母细胞显微注射体外转录的RNA从THT 1基因，他们表达的葡萄糖转运蛋白的性质类似的锥虫血流形式的蛋白质（S）。这种THT 1编码的葡萄糖转运系统与人红细胞型葡萄糖转运蛋白的不同之处在于其对细胞松弛素B的中等敏感性及其转运D-果糖的能力。这些性质表明，锥虫转运蛋白可能是一个很好的抗锥虫药物的目标。mRNA分析表明，这些基因的表达是生活史阶段依赖。血流形式表达的THT 1比THT 2多40倍。相比之下，前环锥虫不表达可检测到的THT 1，但显示THT 2的葡萄糖依赖性表达。
A tandemly arranged multigene family encoding putative hexose transporters in Trypanosoma brucei has been characterized. It is composed of two 80% homologous groups of genes called THT1 (six copies) and THT2 (five copies). When Xenopus oocytes are microinjected with in vitro-transcribed RNA from a THT1 gene, they express a glucose transporter with properties similar to those of the trypanosome bloodstream-form protein(s). This THT1-encoded transport system for glucose differs from the human erythrocyte-type glucose transporter by its moderate sensitivity to cytochalasin B and its capacity to transport D-fructose. These properties suggest that the trypanosomal transporter may be a good target for antitrypanosomal drugs. mRNA analysis revealed that expression of these genes was life cycle stage dependent. Bloodstream forms express 40-fold more THT1 than THT2. In contrast, procyclic trypanosomes express no detectable THT1 but demonstrate glucose-dependent expression of THT2.