Effect of 5-fluorouracil on “primitive” hematopoietic stem cells that reconstitute whole erythropoiesis of genetically anemic W/Wv mice
Effect of 5-fluorouracil on “primitive” hematopoietic stem cells that reconstitute whole erythropoiesis of genetically anemic W/Wv mice
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5-氟尿嘧啶对重建遗传性贫血 W/Wv 小鼠全红细胞生成的“原始”造血干细胞的影响
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Y. Kitamura
中科院分区：
文献类型：
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作者：
T. Nakano;N. Waki;H. Asai;Y. Kitamura
比较了经5-氟尿嘧啶(5FU)处理和未处理的遗传性贫血(WB X C57BL/6)F1-W/WV(WBB6F1-W/WV)小鼠在至少8周内重建整个红细胞生成的潜力。供体为C57BL/6-PGK-1b/PGK-1a雌性小鼠，每只小鼠的干细胞由于两条X染色体中的一条随机失活而具有A型或B型磷酸甘油酸激酶(PGK)。作为重建的标志，使用了血红蛋白的电泳谱。WBB6F1-W/WV小鼠骨髓中重建整个红细胞的干细胞浓度高于未注射5FU两天的供者骨髓中的浓度。然而，在接受5FU治疗4天的小鼠的骨髓中，这种浓度与未治疗的小鼠相当。由未经处理的骨髓细胞重组的WBB6F1-W/WV小鼠的PGK图谱与经5FU处理4天的骨髓细胞重组的WBB6F1-W/WV小鼠的PGK图谱相似。因此，在未经处理的健康骨髓细胞和5FU处理的骨髓细胞的移植中，具有广泛增殖潜力的单个干细胞而不是多个脾集落形成单位似乎负责红细胞重建。
The potential to reconstitute the whole erythropoiesis of a genetically anemic (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mouse for at least 8 weeks was compared between 5-fluorouracil (5FU)-treated and nontreated bone marrow cells. C57BL/6-Pgk-1b/Pgk-1a female mice, in which each stem cell had either A-type or B-type phosphoglycerate kinase (PGK) owing to the random inactivation of one of two X chromosomes, were used as donors. As a marker of the reconstitution, electrophoretic pattern of hemoglobin was used. The concentration of the stem cells that reconstitute the whole erythropoiesis of WBB6F1-W/Wv mouse was higher in the marrow of donors that had received an injection of 5FU two days previously (two-day 5FU-treated) than in the marrow of nontreated donors. In the marrow of four-day 5FU-treated mice, however, the concentration was comparable to that of nontreated mice. The PGK electrophoretic pattern of WBB6F1-W/Wv mice reconstituted by nontreated marrow cells was comparable to the PGK pattern of WBB6F1-W/Wv mice reconstituted by four-day 5FU-treated marrow cells. Thus, a single stem cell with extensive proliferative potential rather than multiple spleen colony-forming units appeared to be responsible for the erythropoietic reconstitution in the transplantation of nontreated healthy marrow cells as well as 5FU-treated marrow cells.