microRNA-375 modulates the NF-κB pathway in miiuy croaker by targeting DUSP1 gene
microRNA-375 modulates the NF-κB pathway in miiuy croaker by targeting DUSP1 gene
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microRNA-375通过靶向DUSP1基因调节黄花鱼的NF-kappa B通路
DOI：
10.1016/j.dci.2018.05.010
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发表时间：
2018-09-01
影响因子：
2.9
通讯作者：
Xu, Tianjun
中科院分区：
文献类型：
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作者：
Sun, Yuena;Chu, Qing;Xu, Tianjun
microRNA（miRNA）是高度保守的非编码小内源分子，可以参与生物体的发育、生长和免疫反应等多种生物过程。双特异性磷酸酶 (DUSP) 是可以从磷酸酶中去除磷酸基团的酶。研究发现DUSP1是MAPK调节过程中的重要分子。然而，DUSP1作为重要的调控因子，在鱼类中的研究却很少。因此，在本研究中，通过双荧光素酶报告基因测定和蛋白质印迹分析验证了miRNA对DUSP1的调节作用。此外，我们发现miR-375模拟物和pre-miR-375质粒可以通过与DUSP1基因的3'非翻译区结合来负向调节小黄花鱼的靶基因DUSP1。这些实验结果直接表明了miR-375对DUSP1的负调控功能。此外，miR-375 可以通过靶向 DUSP1 负向调节 NF-kappa B 信号通路。这项研究可以增加我们的知识，帮助我们了解硬骨鱼基因组的复杂性和复杂的基因表达调控网络。 (C) 2018 Elsevier Ltd. 保留所有权利。
microRNAs (miRNAs) are highly conserved, small non-coding endogenous molecule, and can participate in a variety of biological processes in organisms such as development, growth and immune response. Dual-Specificity Phosphatases (DUSPs) are enzymes that can remove phosphate groups from phosphatases. Research found that DUSP1 is an important molecule in the process of MAPK regulation. However, as a significant regulatory factor, the study of DUSP1 was very few in fish. Consequently, in this study, the regulatory role of miRNAs on DUSP1 has been verified through dual-luciferase reporter assay and western blotting analysis. Furethermore, we found that miR-375 mimics and pre-miR-375 plasmid can negatively regulate the target gene DUSP1 in miiuy croaker through combining with 3'untranslated region of DUSP1 gene. These experiment results directly indicate the negative regulatory function of miR-375 to DUSP1. Moreover, miR-375 can negatively regulate NF-kappa B signaling pathway via target to DUSP1. This study can increase our knowledge and help us to understand complexity of genomic and complex gene expression regulatory networks in teleost fish. (C) 2018 Elsevier Ltd. All rights reserved.