Genotypes and antimicrobial profiles of Shigella sonnei isolates from diarrheal patients circulating in Beijing between 2002 and 2007.
Genotypes and antimicrobial profiles of Shigella sonnei isolates from diarrheal patients circulating in Beijing between 2002 and 2007.
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DOI：
10.1016/j.diagmicrobio.2012.06.026
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发表时间：
2012-10
影响因子：
2.9
通讯作者：
Mao Y
中科院分区：
文献类型：
--
作者：
Qu F;Bao C;Chen S;Cui E;Guo T;Wang H;Zhang J;Wang H;Tang YW;Mao Y
宋内志贺菌是近年来亚洲国家引起志贺菌病的主要血清型。在这项研究中，我们的特点是抗生素耐药谱和基因型的增加趋势。宋内氏菌在北京地区的分离株。2002年1月至2007年12月，共分离志贺菌1108株，其中志贺菌362株。sonnei是从北京302医院的腹泻患者中恢复的。而S. flexneri逐渐减少，S. sonnei逐渐增加并成为优势种。共362个S。进一步分析Sonnei分离株的抗菌谱，并分别选择272个复活的分离株进行基因分型分析。2002 - 2007年，观察到磺胺甲恶唑/甲氧苄啶（94.5%）、氨苄西林（40.3%）、哌拉西林（36.5%）和头孢曲松（12.8%）的高水平耐药，单药和多药耐药趋势显著增加（P = 0.0000）。脉冲场凝胶电泳分析表明，263株（96.7%）S。sonnei属于1个克隆基因型A，进一步分为A1-A6亚型。A2亚型在研究年份的早期占主导地位，而A4亚型开始出现，并在以后的年份中显著增加。6种亚型的耐药率差异有统计学意义（P = 0.0000），其中A4型对氨苄西林和哌拉西林的耐药率最高，分别为83.7%和81.4%。A3亚型与其他亚型相比在住院患者中高度聚集（P = 0.0145）。本研究表明，克隆S。宋内氏菌株已成为北京地区的优势菌株，A4亚型是导致抗生素耐药性增加的原因。
Shigella sonnei has become the dominant serotype causing shigellosis in Asian countries in recent years. In this study, we characterize the increasing trend of antibiotic resistance profiles and genotypes of S. sonnei isolates in the Beijing area. From January 2002 to December 2007, a total of 1108 Shigella isolates including 362 S. sonnei were recovered from diarrhea patients at the 302nd Hospital in Beijing. While the frequency of S. flexneri gradually decreased, S. sonnei gradually increased and became the dominant species. A total of 362 S. sonnei isolates were further analyzed for their antimicrobial profiles and 272 revived isolates were selected for genotyping analysis, respectively. High-level antimicrobial resistances were observed in sulfamethoxazole/trimethoprim (94.5%), ampicillin (40.3%), piperacillin (36.5%), and ceftriaxone (12.8%) with significant single- and multiple-drug resistance increase trends from 2002 to 2007 (P = 0.0000). Pulsed-field gel electrophoresis analysis indicated that 263 (96.7%) S. sonnei belonged to 1 clonal genotype A, which were further divided into A1–A6 subtypes. While subtype A2 was dominant in the early stage of study years, subtype A4 started to emerge and increased significantly in later years. Antimicrobial resistance rates are statistically different among the 6 subtypes (P = 0.0000), and A4 possessed the highest resistance rates to ampicillin (83.7%) and piperacillin (81.4%). Subtype A3 was highly clustered in inpatients compared to other subtypes (P = 0.0145). This study indicates that a clonal S. sonnei strain has become dominant in the Beijing area, and subtype A4 is responsible for increased antibiotic resistance.
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影响因子：
11.8
作者：
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通讯作者：
Casanova, JL
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11.8
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通讯作者：
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作者：
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通讯作者：
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4.2
作者：
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Effler, P. V.