RNA-transfected dendritic cells.
RNA-transfected dendritic cells.
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DOI：
10.1586/14760584.1.4.507
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发表时间：
2002-12-01
影响因子：
6.2
通讯作者：
Boczkowski, David
中科院分区：
文献类型：
--
作者：
Nair, Smita;Boczkowski, David
基于其刺激初级免疫应答的独特能力，树突状细胞是已知的最有效的抗原呈递细胞。这种能力源于这样的事实，即它们在抗原的摄取和加工方面非常有效，并且它们表达高水平的主要组织相容性复合物I类和II类以及共刺激分子，这是引发幼稚细胞毒性T细胞所需的。许多研究小组试图利用这些特征，开发基于树突状细胞的疫苗来对抗肿瘤和传染病。虽然这些研究的基本原理是相同的--用抗原脉冲的树突状细胞来引发细胞毒性T细胞反应--但使用的方法是不同的。对于所使用的抗原的性质和抗原递送方法来说尤其如此。在这篇文章中，我们将集中在使用RNA作为抗原的一种形式与负载树突状细胞。我们将讨论使用RNA作为抗原来源的基本原理，并将回顾最近在小鼠和人类环境中使用RNA脉冲树突状细胞作为疫苗的研究。
Based on their unique ability to stimulate primary immune responses, dendritic cells are the most potent antigen-presenting cells known. This ability stems from the fact that they are very efficient at the uptake and processing of antigen and they express high levels of major histocompatibility complex class I and class II, as well as costimulatory molecules, which are required to prime naive cytotoxic T-cells. Many groups of investigators have tried to take advantage of these features by developing dendritic cell-based vaccines against tumors and infectious diseases. While the basic principle in these studies is the same--dendritic cells pulsed with antigen are used to elicit cytotoxic T-cell responses--the methods used are varied. This is particularly true with respect to the nature of the antigen used and the method of antigen delivery. In this article, we will focus on the use of RNA as a form of antigen with which to load dendritic cells. We will discuss the rationale behind using RNA as an antigen source and will review recent studies in both murine and human settings that use RNA-pulsed dendritic cells as vaccines.