iTRAQ protein profiling reveals candidate proteins regulating ovary and ovule differentiation in pistillate inflorescences after pollination in hazel
iTRAQ protein profiling reveals candidate proteins regulating ovary and ovule differentiation in pistillate inflorescences after pollination in hazel
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iTRAQ 蛋白质分析揭示了榛树授粉后调节雌花序子房和胚珠分化的候选蛋白质
DOI：
10.1007/s11295-019-1328-7
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发表时间：
2019-04-01
影响因子：
2.4
通讯作者：
Liu, Jianfeng
中科院分区：
文献类型：
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作者：
Cheng, Yunqing;Mou, Yao;Liu, Jianfeng
榛（Corylusspp.）具有独特的子房和胚珠发育特征，子房、胚珠和成熟胚囊在授粉后才开始发育。榛子子房和胚珠的频繁发育失败是导致产量损失的主要原因。尽管在经济上的重要性，参与调控卵巢和胚珠分化的蛋白质仍然在很大程度上未知。为了解决这些差距，总蛋白质提取的雌花序或年轻的子房在三个阶段：阶段F（未授粉的花序），阶段S（一个不发育的卵巢形成的开始），和阶段T（胚珠分化）。使用同量异位素标签进行蛋白质谱分析，用于相对和绝对定量（iTRAQ），导致2586个蛋白质的鉴定。总共鉴定了815个差异表达蛋白（DEP），包括S-vs-F、T-vs-S和T-vs-F配对比较中的386、173和697个DEP。使用iTRAQ技术的蛋白质和DEP在mRNA水平上显示出比先前比较转录组分析中的差异表达基因（DEG）和单基因显著更高的转录丰度。途径作图结果显示，9个途径显着丰富，共享两个共同的KEGG途径，ko 00940（苯丙素类生物合成）和ko 00941（类黄酮生物合成），这是以前报道的比较转录组分析。在815个DEPs中，有236个DEPs在mRNA水平上同时差异表达，基于表达和mRNA水平的结果预测了包括查尔酮合成酶在内的一组DEPs参与子房和胚珠的分化。我们的研究结果在表达水平上为深入了解授粉后子房分化和发育的分子机制提供了新的思路。
Hazel (Corylusspp.) has unique ovary and ovule development characteristics, as the ovary, ovule, and mature embryo sac only begin to develop after pollination. Frequent development failure of ovary and ovule causes yield losses in hazelnut. Despite the economic importance, proteins involved in the regulation of ovary and ovule differentiation remain largely unknown. To address these gaps, total protein was extracted from the pistillate inflorescences or young ovaries at three stages: stage F (unpollinated inflorescences), stage S (beginning of a rudimentary ovary formation), and stage T (ovule differentiation). Protein profiling analysis was carried out using isobaric tags for relative and absolute quantitation (iTRAQ), leading to the identification of 2586 proteins. In total, 815 differentially expressed proteins (DEPs) were identified, including 386, 173, and 697 DEPs in S-vs-F, T-vs-S, and T-vs-F paired comparisons, respectively. The proteins and DEPs using iTRAQ technology showed substantially higher transcript abundance than differentially expressed genes (DEGs) and unigenes in previous comparative transcriptome analysis at mRNA level. Pathway mapping results revealed that nine pathways were significantly enriched, sharing two common KEGG pathways, ko00940 (phenylpropanoid biosynthesis) and ko00941 (flavonoid biosynthesis), which were reported previously in a comparative transcriptome analysis. Of 815 DEPs, 236 were simultaneously differentially expressed at mRNA level, and a set of DEPs including chalcone synthase was predicted to participate in ovary and ovule differentiation based on the results at expression and mRNA level. Our findings provide new insight into the molecular mechanisms of ovary differentiation and development after pollination at the expression level.