Expression of extracellular matrix molecules, MMPs and TIMPs in alveolar bone, cementum and periodontal ligaments during rat tooth eruption.
Expression of extracellular matrix molecules, MMPs and TIMPs in alveolar bone, cementum and periodontal ligaments during rat tooth eruption.
复制标题
大鼠牙齿萌出过程中牙槽骨、牙骨质和牙周膜中细胞外基质分子、MMPs和TIMPs的表达。
DOI：
10.1093/jmicro/52.6.593
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
H. Mayanagi
中科院分区：
文献类型：
--
作者：
Y. Maruya;Y. Sasano;I. Takahashi;M. Kagayama;H. Mayanagi
牙齿萌出涉及细胞外基质（ECM）成分的广泛降解和重组。目前尚不清楚ecm降解酶是如何相互协调的，也不知道在这种情况下它们是如何被调节的。本研究旨在利用原位杂交技术研究大鼠第一磨牙出疹过程中金属蛋白酶抑制剂（TIMPs）与基质金属蛋白酶（MMPs）和ECM分子的mRNA表达。我们还研究了TIMPs如何参与牙齿萌牙、牙根形成、牙骨质发生和牙槽骨重塑的过程。在成骨细胞、骨细胞、成骨水泥细胞、成骨水泥细胞和牙周韧带成纤维细胞中可见i型胶原、骨钙素、MMPs 2和8以及TIMPs 1、2和3的表达，在第一磨牙出牙中期，在牙槽骨、牙骨质和牙周韧带中可见ECM分子、MMPs和TIMPs的高表达。牙周组织中ECM的重塑可能通过ECM分子的产生、MMPs对ECM的降解和TIMPs对MMPs的抑制之间的平衡来调节。
Tooth eruption involves extensive degradation and reorganization of extracellular matrix (ECM) components. It is not known how ECM-degrading enzymes are coordinated with each other or how they are regulated in the event. The present study was designed to investigate mRNA expression of inhibitors of metalloproteinases (TIMPs) in comparison with matrix metalloproteinases (MMPs) as well as ECM molecules during rat first molar eruption using in situ hybridization. We also examined how TIMPs are involved in the process of tooth eruption, root formation, cementogenesis and alveolar bone remodelling. Expressions of type-I collagen, osteocalcin, MMPs 2 and 8, and TIMPs 1, 2 and 3 were shown in osteoblasts, osteocytes, cementoblasts, cementocytes and periodontal ligament fibroblasts, and the concomitant high expressions of the ECM molecules, MMPs and TIMPs in alveolar bone, cementum and periodontal ligaments were identified in the middle of first molar eruption. The remodelling of ECM in these periodontal tissues might be regulated through balance among the production of ECM molecules, the degradation of ECM by MMPs and the inhibition of MMPs by TIMPs during tooth eruption.