Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts.
Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts.
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DOI：
10.1111/j.1751-7915.2010.00246.x
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发表时间：
2011-07
影响因子：
5.7
通讯作者：
Cowan DA
中科院分区：
文献类型：
--
作者：
Taylor MP;van Zyl L;Tuffin IM;Leak DJ;Cowan DA
从生物质可持续地生产生物产品的环境价值现在被广泛地认识到，其中主要目标是从木质纤维素经济地生产燃料如生物乙醇。嗜热原核生物的应用是该领域中快速发展的利基，这是由其已知的与木质纤维素衍生的碳水化合物的分解代谢多功能性驱动的。这项工作取得成功的基础是开发可靠的遗传和分子系统。这些技术工具现在可用于帮助开发具有不同表型的其他（超）嗜热菌株，如半纤维素分解和纤维素分解特性，支链醇生产和其他“有价值的生物产品”合成能力。在这里，我们提出了一个洞察到的历史局限性，最近的发展和一些嗜热菌遗传系统的现状。我们还强调了可靠的遗传方法的价值，以增加我们的嗜热生理学知识。我们认为，强大的遗传系统的发展是至关重要的，在未来的嗜热生物过程的演变，并提出建议，为未来的方法和遗传目标，将促进这一进程。
The environmental value of sustainably producing bioproducts from biomass is now widely appreciated, with a primary target being the economic production of fuels such as bioethanol from lignocellulose. The application of thermophilic prokaryotes is a rapidly developing niche in this field, driven by their known catabolic versatility with lignocellulose‐derived carbohydrates. Fundamental to the success of this work has been the development of reliable genetic and molecular systems. These technical tools are now available to assist in the development of other (hyper)thermophilic strains with diverse phenotypes such as hemicellulolytic and cellulolytic properties, branched chain alcohol production and other ‘valuable bioproduct’ synthetic capabilities. Here we present an insight into the historical limitations, recent developments and current status of a number of genetic systems for thermophiles. We also highlight the value of reliable genetic methods for increasing our knowledge of thermophile physiology. We argue that the development of robust genetic systems is paramount in the evolution of future thermophilic based bioprocesses and make suggestions for future approaches and genetic targets that will facilitate this process.
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影响因子：
2.6
作者：
DEROSSI, E;BRIGIDI, P;MATTEUZZI, D
通讯作者：
MATTEUZZI, D
影响因子：
3.2
作者：
Frigaard, NU;Maresca, JA;Bryant, DA
通讯作者：
Bryant, DA
影响因子：
3.2
作者：
Erauso, G;Marsin, S;Forterre, P
通讯作者：
Forterre, P
影响因子：
4.4
作者：
Friedrich, A;Rumszauer, J;Averhoff, B
通讯作者：
Averhoff, B
影响因子：
5
作者：
Bjornsdottir, SH;Thorbjarnardottir, SH;Eggertsson, G
通讯作者：
Eggertsson, G