High temperature exposure did not affect induced 2n pollen viability in Populus
High temperature exposure did not affect induced 2n pollen viability in Populus
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高温暴露不影响胡杨诱导的 2n 花粉活力
DOI：
10.1111/pce.13165
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发表时间：
2018-06-01
影响因子：
7.3
通讯作者：
Zhang, Pingdong
中科院分区：
文献类型：
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作者：
Tian, Mengdi;Zhang, Yuan;Zhang, Pingdong
高温处理是杨树2n配子体诱导的一种常用的物理诱变剂。然而，高温暴露是否影响诱导的2n花粉生活力仍然未知。为了阐明高温暴露是否影响诱导的2n花粉生活力，由38和41摄氏度的温度，花粉形态，2n花粉在体外萌发，和杂交诱导的2n花粉与正常配子产生一个三倍体的诱导的2n花粉诱导的2n花粉，减数分裂的观察的基础上，在白杨进行。结果表明，减数分裂的优势时期（F = 56.6，p <0.001）和处理时间（F=21.4，p <0.001）显著影响诱导2n花粉的发生率。观察到花粉产量显著减少，败育花粉增加（p <0.001）。高温有时会影响外凝沉积，并通过外凝结构的细节分析了一些狭窄的犁沟。而自然2n花粉的萌发率（26.7%）与高温诱导的2n花粉的萌发率（26.2%）无显著差异，高温诱导的2n花粉杂交产生了42个三倍体，表明38 ℃和41 ℃的高温暴露不会导致诱导的2n花粉功能失调。
High temperature exposure is widely used as a physical mutagenic agent to induce 2n gametes in Populus. However, whether high temperature exposure affects induced 2n pollen viability remains unknown. To clarify whether high temperature exposure affected the induced 2n pollen viability, 2n pollen induced by 38 and 41 degrees C temperatures, pollen morphology, 2n pollen germination in vitro, and crossing induced 2n pollen with normal gametes to produce a triploid was, based on observations of meiosis, conducted in Populus canescens. We found that the dominant meiotic stages (F = 56.6, p < .001) and the treatment duration (F=21.4, p < .001) significantly affected the occurrence rate of induced 2n pollen. A significant decrease in pollen production and an increase in aborted pollen were observed (p < .001). High temperature sometimes affected in ectexine deposition and some narrow furrows were also analysed via details of ectexine structure. However, no significant difference in 2n pollen germination rate was observed between natural 2n pollen (26.7%) and high-temperature-induced 2n pollen (26.2%), and 42 triploids were created by crossing high-temperature-induced 2n pollen, suggesting that 38 and 41 degrees C temperatures exposure will not result in dysfunctional induced 2n pollen.