Methotrexate-conjugated magnetic nanoparticles for thermochemotherapy and magnetic resonance imaging of tumor
Methotrexate-conjugated magnetic nanoparticles for thermochemotherapy and magnetic resonance imaging of tumor
复制标题
甲氨蝶呤偶联的磁性纳米粒子用于肿瘤热化疗和磁共振成像
DOI：
10.1007/s11051-012-1160-6
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发表时间：
2012-10-01
影响因子：
2.5
通讯作者：
Tang, Jintian
中科院分区：
文献类型：
--
作者：
Gao, Fuping;Yan, Zixing;Tang, Jintian
近年来，在开发具有互补作用的多功能特性的磁性纳米颗粒（MNP）方面存在显著的兴趣。在这项研究中，甲氨蝶呤（MTX）的铁氧化物磁性纳米粒子的表面共轭通过聚（乙烯亚胺）自组装单分子层（MTX-MNP）。该新平台结合了癌症化疗，热疗和通过磁共振成像（MRI）监测疾病进展的潜力。通过傅里叶变换红外光谱和ζ电位的变化证实了MTX在磁铁矿表面的共轭作用。透射电子显微镜（TEM）显示MTX-MNP为球形。动态光散射法测得MTX-MNP的平均粒径为30.1 ± 5.2nm。磁性测试结果表明，MTX-MNP的饱和磁化强度达到68.8emu/g，具有超顺磁性。在交变磁场中，MTX-MNP具有良好的加热性能。透射电镜结果显示，与MNP相比，更多的MTX MNP被内化到MCF-7细胞的细胞质中。MTX-MNP在MCF-7乳腺癌细胞中表现出同时化疗和高温的高度协同抗增殖作用。与L929细胞相比，当两者都与纳米缀合物一起培养时，用磁共振体模成像观察到MCF-7细胞的显著负对比度增强。具有热化疗和MRI结合特征的MTX MNP在肿瘤治疗中具有较高的临床意义。
There is significant interest in recent years in developing magnetic nanoparticles (MNPs) having multifunctional characteristics with complimentary roles. In this study, methotrexate (MTX) was conjugated on the iron oxide magnetic nanoparticles surface via a poly(ethyleneimine) self-assembled monolayer (MTX–MNPs). The novel platform combined cancer chemotherapy, hyperthermia and potential monitoring of the progression of disease through magnetic resonance imaging (MRI). The conjugation of MTX on the magnetite surface was confirmed by Fourier transform infrared spectroscopy and change of zeta potential. Transmission electron microscope (TEM) showed that MTX–MNPs were morphologically spherical. The average diameter of MTX–MNPs was 30.1 ± 5.2 nm determined by dynamic light scattering. Magnetic measurements revealed that the saturation magnetization of MTX–MNPs reached 68.8 emu/g and the nanoparticles were superparamagnetic. The MTX–MNPs had good heating properties in an alternating magnetic field. TEM results showed that a larger number of MTX–MNPs were internalized into the MCF-7 cellular cytoplasm compared with the MNPs. The MTX–MNPs demonstrated highly synergistic antiproliferative effects of simultaneous chemotherapy and hyperthermia in MCF-7 breast cancer cells. A significant negative contrast enhancement was observed with magnetic resonance phantom imaging for MCF-7 cells over L929cells, when both were cultured with the nanoconjugate. The MTX–MNPs with combined characteristics of thermochemotherapy and MRI could be of high clinical significance in the treatment of tumor.