Tropism of bunyaviruses: evidence for a G1 glycoprotein-mediated entry pathway common to the California serogroup.
Tropism of bunyaviruses: evidence for a G1 glycoprotein-mediated entry pathway common to the California serogroup.
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布尼亚病毒的趋向性：加州血清群常见的 G1 糖蛋白介导的进入途径的证据。
DOI：
10.1006/viro.1995.0043
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Gonzalez-Scarano,F
中科院分区：
文献类型：
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作者：
Pekosz,A;Griot,C;Nathanson,N;Gonzalez-Scarano,F
加利福尼亚血清群由布尼亚病毒科布尼亚病毒属内的抗原性和生物学上相关的病毒组成。我们使用了一大组小鼠细胞来研究它们的组织趋向性，发现这个血清组的所有成员与该家族的其他成员(Bunyamwera、高速缓存谷和Punta Toro病毒)的病毒复制模式几乎相同。通过用RNA斑点杂交和病毒结合试验分析非许可感染，我们确定培养细胞的嗜性是在进入水平上决定的。La Crosse G1糖蛋白(SG1)的一种截短的可溶性形式在杆状病毒系统中表达，尽管糖基化略有不同，但通过六种单抗的免疫沉淀显示与天然的G1相似。流式细胞仪显示，SG1与许可细胞系结合，但不与非许可细胞系结合。SG1有效地阻止了所有加州血清组病毒对允许细胞系的感染，但没有阻止另外两种布尼亚病毒的感染。这些结果表明，加州血清群布尼亚病毒在脊椎动物细胞上共享一个共同的受体，该受体可能不同于其他布尼亚病毒科使用的受体，并表明G1糖蛋白是病毒附着蛋白。在寻找可能的受体分子方面，SG1将是一个有用的试剂。
The California serogroup is composed of antigenically and biologically related viruses within theBunyavirusgenus of the Bunyaviridae. We used a large panel of murine cells to study their tissue tropisms and found virtually identical patterns of viral replication among all of the members of this serogroup, in contrast to other members of the family (Bunyamwera, Cache Valley, and Punta Toro viruses). By analyzing the nonpermissive infections with both an RNA dot-blot and a virus binding assay, we determined that tropism for cultured cells was determined at the level of entry. A truncated soluble form of the La Crosse G1 glycoprotein (sG1) was expressed in a baculovirus system and, despite slight differences in glycosylation, was shown to resemble native G1 by immunoprecipitation with six monoclonal antibodies. sG1 bound to permissive but not to nonpermissive cell lines, as demonstrated by flow cytometry. The sG1 effectively blocked infection of permissive cell lines with all of the California serogroup viruses, but did not block infection of two other bunyaviruses. These results indicate that the California serogroup bunyaviruses share a common receptor on vertebrate cells which may differ from the receptor used by other Bunyaviridae and demonstrate that the G1 glycoprotein is the virus attachment protein. sG1 will be a useful reagent in the search for a putative receptor molecule.