Synthesis of Acetylene Linked Double-Nucleobase Nucleos(t)ide Building Blocks and Polymerase Construction of DNA Containing Cytosines in the Major Groove
Synthesis of Acetylene Linked Double-Nucleobase Nucleos(t)ide Building Blocks and Polymerase Construction of DNA Containing Cytosines in the Major Groove
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DOI：
10.1021/jo200436j
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发表时间：
2011-05-06
影响因子：
3.6
通讯作者：
Hocek, Michal
中科院分区：
文献类型：
--
作者：
Kielkowski, Pavel;Pohl, Radek;Hocek, Michal
嘧啶和7 - 脱氮腺嘌呤2 - 脱氧核糖核苷以及核苷三磷酸（dNTPs）的（胞嘧啶 - 5 - 基）乙炔基衍生物通过未保护的卤代核苷（苷酸）与5 - 乙炔基胞嘧啶在水溶液中进行Sonogashira偶联一步制备而成。修饰后的dNTPs是适合用于引物延伸或通过聚合酶链式反应（PCR）构建在大沟中含有模拟翻转出来的核苷酸的乙炔连接胞嘧啶的DNA的DNA聚合酶的良好底物。
(Cytosin-5-yl)ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared in one step by the aqueous Sonogashira coupling of unprotected halogenated nucleos(t)ides with 5-ethynylcytosine. The modified dNTPs were good substrates for DNA polymerases suitable for primer extension or PCR construction of DNA bearing acetylene-linked cytosine(s) in the major groove mimicking the flipped-out nucleotide.