Expression analysis of microRNAs and their target genes during experimental diabetic renal lesions in rats administered with ginsenoside Rb1 and trigonelline
Expression analysis of microRNAs and their target genes during experimental diabetic renal lesions in rats administered with ginsenoside Rb1 and trigonelline
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人参皂苷Rb1和葫芦巴碱大鼠实验性糖尿病肾损伤过程中microRNA及其靶基因的表达分析
DOI：
10.1691/ph.2019.8903
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发表时间：
2019-08-01
期刊：
影响因子：
1.6
通讯作者：
Shi, Yan
中科院分区：
文献类型：
--
作者：
Shao, Xiaona;Chen, Chen;Shi, Yan
目的：目的：评价黄芪皂苷Rb 1和胡芦巴碱对糖尿病肾病的治疗作用，并对实验性糖尿病肾病大鼠肾脏病变过程中microRNA及其靶基因的表达进行分析。方法：Wistar大鼠腹腔注射链脲佐菌素（STZ）55 mg/kg，造成糖尿病模型。根据空腹血糖值和初始体重，将糖尿病大鼠分为特定组，并处理如下：DN组（自来水，n = 10），A组（人参皂苷Rb 1，40 mg/kg，n = 10），B组C组（胡芦巴碱+Rb 1，60 mg/kg，m（Rb 1）：m（胡芦巴碱）= 2：1，n = 10）。对照组10例，给予自来水治疗。所有大鼠每天灌胃药物或自来水一次，连续12周。结果如下：联合应用Rb 1和胡芦巴素（C组）可明显减轻肾功能不全、氧化应激和病理改变。在Con、DN、A、C组中有部分miRNAs表达差异。免疫组化和Western blotting结果显示，Con组、DN组和C组Wnt和β-catenin的表达存在差异。结论：人参皂苷Rb 1和胡芦巴碱可通过调节miR-3550的表达，进而与Wnt/beta-catenin信号通路相关，从而预防糖尿病肾脏病变的发生。
Purpose: To appraise the curative effect of ginsenoside Rb1 and trigonelline in diabetic nephropathy and to analyze the expression analysis of microRNAs and their target genes during experimental diabetic renal lesions in rats. Methods: Wistar rats were made diabetic by intraperitoneal injection of 55 mg/kg streptozotocin. According to their fasting blood glucose values and initial body weight, diabetic rats were assigned to specific groups and treated as follows: DN group (tap water, n = 10), A group (ginsenoside Rb1, 40 mg/kg, n = 10), B group (trigonelline, 20 mg/kg, n = 10) and the C group (ginsenoside Rb1 and trigonelline, 60 mg/kg, m(ginsenoside Rb1) : m (trigonelline) = 2:1, n = 10). The control group was treated with tap water (n = 10). All rats were gavaged with drugs or tap water once daily for 12 weeks. Results: Renal dysfunction, oxidative stress, and pathological alteration were significantly alleviated by a combination of ginsenoside Rb1 and trigonellin (C group). Some miRNAs were expressed differentially in Con, DN, A and C groups. Results of immunohistochemistry and western blotting showed that Wnt and p-catenin were expressed differentially in Con, DN, and C groups. Conclusion: Ginsenoside Rb1 and trigonelline could prevent the development of diabetic renal lesions by regulating the expression of miR-3550 and further associating with the Wnt/beta-catenin signaling.