Urine culture contamination: a College of American Pathologists Q-Probes study of contaminated urine cultures in 906 institutions.
Urine culture contamination: a College of American Pathologists Q-Probes study of contaminated urine cultures in 906 institutions.
复制标题
尿培养污染：美国病理学家学院 Q-Probes 对 906 家机构的受污染尿培养进行的研究。
DOI：
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发表时间：
1998
影响因子：
4.6
通讯作者：
F. Meier
中科院分区：
文献类型：
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作者：
P. Valenstein;F. Meier
目标
检查门诊患者尿培养污染的频率和原因。
方法
906 个机构的规模从不足 50 张床位到超过 600 张床位不等，每个机构连续检查了门诊患者的 250 份尿液培养物，并鉴定出符合研究污染定义的标本。参与者还回答了有关收集、运输和处理尿液样本的做法的问题。总共分析了超过 200,000 份尿液培养物的相关数据。
结果
中位机构报告称，从门诊采集的尿培养标本中有18.1%受到污染。排名前 10% 的机构（第 90%）报告称，其培养物中 5.6% 或更少受到污染。相比之下，排名最后 10% 的机构（第 10 个百分位）报告称，他们的尿液样本中有 36.8% 或更多受到污染。污染率较低的机构往往处理较少比例的女性患者标本。儿科医院的儿童标本污染率也低于综合医院。在多变量分析中，其他因素，包括使用中央处理区、冷藏、尿液筛查系统、标本防腐剂、提供书面采集说明或特殊采集套件以及隔热标本运输容器，均未发现与低标本污染率相关。
结论
门诊尿培养污染是一种常见现象，不同设施的尿培养污染总体频率存在显着差异。许多通常被认为可以减少污染的干预措施在本研究中并未被证明有效。
OBJECTIVE
To examine the frequency and causes of urine culture contamination in outpatients.
METHODS
Nine hundred six institutions, ranging in size from less than 50 to more than 600 beds, each examined 250 consecutively ordered urine cultures from outpatients and identified specimens that met the study definition of contamination. Participants also answered questions about the practices used to collect, transport, and process urine specimens. In all, data relating to more than 200,000 urine cultures were analyzed.
RESULTS
The median institution reported that 18.1% of urine cultures specimens collected from outpatients were contaminated. The top 10% of institutions (90th percentile) reported that 5.6% or fewer of their cultures were contaminated. In contrast, the bottom 10% of institutions (10th percentile) reported that 36.8% or more of their urine specimens were contaminated. Institutions with lower contamination rates tended to process a lower proportion of specimens from female patients. Pediatric hospitals also reported lower contamination rates in specimens from children than general hospitals. Other factors, including the use of central processing areas, refrigeration, urine screening systems, specimen preservatives, provision of written collection instructions or special collection kits, and thermally insulated specimen transport containers, were not found to be associated with low specimen contamination rates in a multivariate analysis.
CONCLUSION
Contamination of outpatient urine cultures is a common occurrence, and facilities differ significantly in their overall frequency of urine culture contamination. Many interventions commonly assumed to reduce contamination were not demonstrably effective in this study.