Detection of Koi Herpesvirus in Koi Cyprinus carpio in Taiwan
Detection of Koi Herpesvirus in Koi Cyprinus carpio in Taiwan
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DOI：
10.3147/jsfp.39.109
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发表时间：
2004-06
期刊：
影响因子：
0.6
通讯作者：
C. Tu;Mingqing Weng;J. Shiau;Shih-Yuh Lin
中科院分区：
文献类型：
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作者：
C. Tu;Mingqing Weng;J. Shiau;Shih-Yuh Lin
摘要2002年，台湾发生了一次2岁锦鲤（Cyprinus carpio）的大规模死亡事件。受影响的鱼没有显示任何外部迹象，除了肿胀的鳃。在鳃中观察到一致的组织学变化：上皮细胞增生，嗜酸性颗粒细胞浸润和次级层融合。阴性核衣壳呈二十面体，直径112 μ m。利用锦鲤疱疹病毒（KHV）特异性引物，对锦鲤疱疹病毒（KHV）进行了PCR检测，扩增产物序列与文献报道的序列同源性为99%。这是台湾第一次报告KHV感染。
Mass mortality occurred in 2-year-old koi (fancy carp) Cyprinus carpio in Taiwan in 2002. Affected fish did not show any external signs except swollen gills. The consistent histological changes were observed in the gills;hyperplasia of epithelial cells, infiltration of eosinophilic granular cells and fusion of the secondary lamellae. Negatively stained nucleocapsids were icosahedral and 112p1nm in diameter. Koi herpesvirus (KHV) was detected in the diseased fish by PCR using specific primers for KHV, and sequence of the amplicon showed a 99% identity with the published data. This is the first report of KHV infection in Taiwan.