Target sites for the transposition of rat long interspersed repeated DNA elements (LINEs) are not random.
Target sites for the transposition of rat long interspersed repeated DNA elements (LINEs) are not random.
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大鼠长散布重复 DNA 元件 (LINE) 转座的目标位点不是随机的。
DOI：
10.1093/nar/14.9.3717
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发表时间：
1986
影响因子：
14.9
通讯作者：
D'Ambrosio,E
中科院分区：
文献类型：
--
作者：
Furano,AV;Somerville,CC;Tsichlis,PN;D'Ambrosio,E
大鼠的长散布重复DNA家族（LINE或LIRn家族）包含约40，000个6.7-内切酶（kb）长成员（1）。LINE成员目前可能是移动的，因为它们的存在或缺失会导致三个单拷贝基因座的等位基因变异（2，3）：胰岛素1、莫洛尼白血病病毒整合2（Mlvi-2）（4）和免疫球蛋白重链（Igh）。为了表征LINE插入的靶位点，我们比较了未被占据的Mlvi-2靶位点、其含LINE等位基因和其他几个含LINE位点的DNA序列。尽管总体上不同源，但靶位点共有三个特征：首先，根据位点，它们为68%至86%（A + T），而总大鼠DNA为58%（A + T）（5）。根据位点的不同，一个7 - 15-bp的靶位点序列被复制并位于插入的LINE成员的侧翼。第二个是六核苷酸TACTCA的一种形式（0或1个错配），它也存在于LINE成员中，位于LINE成员富含A的右端之前的高度保守区域。第三个是交替嘌呤/嘧啶（PQ）的延伸。不同LINE成员的富含A的右端在长度和组成上不同，并且特别长的一个的序列表明它包含来自先前转座的富含A的靶位点。
The long interspersed repeated DNA family of rats (LINE or LIRn family) contains about 40,000 6.7-kilobase (kb) long members (1). LINE members may be currently mobile since their presence or absence causes allelic variation at three single copy loci (2, 3): insulin 1, Moloney leukemia virus integration 2 (Mlvi-2) (4), and immunoglobulin heavy chain (Igh). To characterize target sites for LINE insertion, we compared the DNA sequences of the unoccupiedMlvi-2target site, its LINE-containing allele, and several other LINE-containing sites. Although not homologous overall, the target sites share three characteristics: First, depending on the site, they are from 68% to 86% (A+T) compared to 58% (A+T) for total rat DNA (5). Depending on the site, a 7- to 15-bp target site sequence becomes duplicated and flanks the inserted LINE member. The second is a version (0 or 1 mismatch) of the hexanucleotide, TACTCA, which is also present in the LINE member, in a highly conserved region located just before the A-rich right end of the LINE member. The third is a stretch of alternating purine/pyrimidine (PQ). The A-rich right ends of different LINE members vary in length and composition, and the sequence of a particularly long one suggests that it contains the A-rich target site from a previous transposition.