AN INDUCIBLE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI
AN INDUCIBLE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI
复制标题
DOI：
10.1126/science.1683004
复制
发表时间：
1991-11-01
期刊：
影响因子：
56.9
通讯作者：
SCHOOLNIK, GK
中科院分区：
文献类型：
--
作者：
GIRON, JA;HO, ASY;SCHOOLNIK, GK
肠病性大肠杆菌 (EPEC) 是儿童腹泻的原因之一，它以粘附细菌的菌落形式生长在小肠表面和培养的上皮细胞上。当在含有血液的固体培养基上繁殖或附着在 HEp-2 细胞上时，EPEC 会表达绳状的细丝束，称为成束菌毛 (BFP)，从而形成将各个生物体结合在一起的纤维网络。发现 BFP 由 5 个 EPEC 血清群表达，每个血清群都含有一个大约 92 kb 的质粒，此前已知该质粒对人类毒力很重要。当其中两个菌株去除该质粒后，它们既不表达 BFP，也不生长为贴壁菌落。 BFP 抗血清降低了 EPEC 感染培养的上皮细胞的能力。 BFP由19,500道尔顿的重复亚基组成，该亚基的氨基末端氨基酸序列与霍乱弧菌毒素共调控的菌毛蛋白同源。
Enteropathogenic Escherichia coli (EPEC), a cause of childhood diarrhea, grow on the surface of the small intestine and on cultured epithelial cells as colonies of adherent bacteria. When propagated on solid medium containing blood or attached to HEp-2 cells, EPEC express ropelike bundles of filaments, termed bundle-forming pili (BFP), that create a network of fibers that bind together the individual organisms. BFP were found to be expressed by five EPEC serogroups, each harboring a approximately 92-kilobase plasmid previously known to be important for virulence in humans. When two of these strains were cured of this plasmid, they neither expressed BFP nor grew as adherent colonies. An antiserum to BFP reduced the capacity of EPEC to infect cultured epithelial cells. BFP are composed of a repeating subunit of 19,500 daltons, the amino-terminal amino acid sequence of this subunit is homologous to that of the toxin-coregulated pilin of Vibrio cholerae.