Expression of the receptor protein tyrosine kinase myk-1/htk in normal and malignant mammary epithelium
Expression of the receptor protein tyrosine kinase myk-1/htk in normal and malignant mammary epithelium
复制标题
DOI：
10.1006/bbrc.1996.1442
复制
发表时间：
1996-09-24
影响因子：
3.1
通讯作者：
Crompton, MR
中科院分区：
文献类型：
--
作者：
Berclaz, G;Andres, AC;Crompton, MR
我们最近报道了一种新的小鼠受体PTK（myk-1）的分子特征，属于eph-related家庭，其表达差异调节乳腺发育过程中，并在侵袭性小鼠乳腺肿瘤升高。在这篇文章中，我们通过原位杂交和RNA酶保护研究了myk-1表达的细胞起源。小鼠反义myk-1探针特异性识别静息小鼠乳腺中的导管和肺泡上皮。在正常人乳腺中，人类同源物htk的表达也仅限于分泌腔上皮细胞。从人乳房缩小术组织制备的富集的管腔和肌上皮细胞的RNA酶保护分析证实了管腔特异性。在几种人乳腺癌细胞系以及原发性、高级别、浸润性乳腺导管癌中发现htk表达升高。myk-1/htk受体PTK的特异性上皮表达表明这种Eph相关的PTK在成人分泌上皮的分化和/或维持中的专门作用。(C)出版社：Academic Press，Inc.
We have recently reported the molecular characterization of a novel murine receptor PTK (myk-1), belonging to the eph-related family, whose expression was differentially regulated during mammary gland development and elevated in invasive mouse mammary tumours. In this communication we have investigated the cellular origin of myk-1 expression by in situ hybridisation and RNase protection. Murine antisense myk-1 probe specifically recognised ductal and alveolar epithelium in the resting mouse mammary gland. In normal human breast, the expression of htk, the human homologue, was also confined to the secretory luminal epithelial cells. RNase protection analysis of enriched luminal and myoepithelial cells prepared from human reduction mammoplasty tissue confirmed the luminal specificity. Elevated expression of htk was found in several human breast carcinoma cell lines as well as in primary, high grade, infiltrating, ductal carcinomas of the breast. The specific epithelial expression of the myk-1/htk receptor PTK suggests a specialised role of this eph-related PTK in the differentiation and/or maintenance of secretory epithelium in the adult. (C) 1996 Academic Press, Inc.