Biodegradable Hydrogels for Time-Controlled Release of Tethered Peptides or Proteins
Biodegradable Hydrogels for Time-Controlled Release of Tethered Peptides or Proteins
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DOI：
10.1021/bm901235g
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发表时间：
2010-02-01
影响因子：
6.2
通讯作者：
Goepferich, Achim
中科院分区：
文献类型：
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作者：
Brandl, Ferdinand;Hammer, Nadine;Goepferich, Achim
将原料药捆绑在凝胶网络上是控制基于水凝胶的药物递送系统释放动力学的有效方法。在这里，我们报道了原位形成的可生物降解的水凝胶，它允许多肽或蛋白质的共价附着。以支链聚乙二醇为原料，采用步长聚合法制备了水凝胶。凝胶强度在1075 ~ 2435 Pit之间，降解时间在24 ~ 120 h之间。光漂白后的荧光恢复显示，荧光标记的牛血清白蛋白（FITC-BSA）在凝胶形成过程中成功结合到凝胶网络上。在168 h内，由于聚合物降解，拴拴分子的迁移率逐渐增加。得到的释放曲线呈s形，与降解曲线吻合较好。蛋白释放在96 h后完成
Tethering drug substances to it gel network is all effective way of controlling the release kinetics of hydrogel-based drug delivery systems Here, we report oil in situ forming, biodegradable hydrogels that allow for the covalent attachment of peptides or proteins. Hydrogels were prepared by step-growth polymerization of branched poly(ethylene glycol). The gel strength ranged from 1075 to 2435 Pit: the degradation time varied between 24 and 120 h. Fluorescence recovery after photobleaching showed that fluorescently labeled bovine serum albumin (FITC-BSA) was Successfully bound to the gel network during gel formation Within 168 h, the mobility of the tethered molecules gradually increased due to polymer degradation Using FITC-BSA and lysozyme as model proteins, we showed the potential of the developed hydrogels for time-controlled release. The obtained release profiles had a sigmoidal shape and matched the degradation profile very well. protein release wits complete after 96 h