SITE OF PARAMYOSIN IN INSECT FLIGHT-MUSCLE AND PRESENCE OF AN UNIDENTIFIED PROTEIN BETWEEN MYOSIN-FILAMENTS AND Z-LINE
SITE OF PARAMYOSIN IN INSECT FLIGHT-MUSCLE AND PRESENCE OF AN UNIDENTIFIED PROTEIN BETWEEN MYOSIN-FILAMENTS AND Z-LINE
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DOI：
10.1016/0022-2836(77)90163-2
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发表时间：
1977-01-01
影响因子：
5.6
通讯作者：
LUKE, BM
中科院分区：
文献类型：
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作者：
BULLARD, B;HAMMOND, KS;LUKE, BM
用抗副肌球蛋白抗体标记昆虫飞行肌原纤维，并通过荧光显微镜和电镜观察，确定了副肌球蛋白在昆虫飞行肌中的位置。蜣螂副肌球蛋白的抗血清具有对另一种蛋白质以及副肌球蛋白的抗体。特异性抗副肌球蛋白结合到显示副肌球蛋白的Lethocerus肌原纤维的H区仅在该区域暴露。另一种蛋白质的抗体结合在A带的末端。暴露的抗原位点在2个区域的肌原纤维依赖于收缩的肌原纤维的程度：在A带的末端的网站是最暴露在休息长度的肌原纤维和那些在H区缩短的。在拉伸的蜜蜂肌肉中的抗体标记表明，在肌节末端的蛋白质从肌球蛋白丝延伸到Z线。昆虫飞行肌的高静止弹性和振荡收缩能力可能是由于肌球蛋白丝和Z线之间的蛋白质。[Lethocerus cordofanus，L. maximus，Pachnoda ephippiata，Xylocopa flavo-rufa和Heliocopris dilloni.]。
The position of paramyosin in insect flight muscle was determined by labeling myofibrils with antibody to paramyosin and examining them by fluorescent microscopy and EM. Antiserum to dung beetle paramyosin had antibodies to another protein as well as to paramyosin. Specific anti-paramyosin bound to the H-zone of Lethocerus myofibrils showing paramyosin was exposed only in that region. Antibodies to the other protein bound at the ends of the A-band. The exposure of antigenic sites in the 2 regions of the myofibril depended on the extent of contraction in the myofibril: the sites at the end of the A-band were most exposed in rest-length myofibrils and those at the H-zone in shortened ones. Antibody-labeling in stretched bee muscle showed that the protein at the ends of the sarcomere extended from myosin filaments to Z-line. The high resting elasticity of insect flight muscle and hence its capacity for oscillatory contraction may be due to the protein between myosin filaments and Z-line. [Lethocerus cordofanus, L. maximus, Pachnoda ephippiata, Xylocopa flavo-rufa and Heliocopris dilloni.].