MicoRNA-425-5p is a potential prognostic biomarker for cervical cancer
MicoRNA-425-5p is a potential prognostic biomarker for cervical cancer
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DOI：
10.1177/0004563216649377
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Mu, Ning
中科院分区：
文献类型：
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作者：
Sun, Liwei;Jiang, Rong;Mu, Ning
背景：MicroRNA 与肿瘤发生的许多生物学途径有关，并且可以作为许多癌症类型的预后生物标志物。本研究旨在评估miR-4255p在宫颈癌中的预后意义。方法：采用实时聚合酶链反应检测35对宫颈癌组织及其匹配的正常组织以及40例宫颈癌患者、13例良性宫颈疾病患者和32例健康对照的血清样本中miR-425-5p的表达水平。研究了组织和血清中 miR-425-5p 表达水平与临床病理因素之间的关联。采用Kaplan-Meier分析和Cox比例风险模型评估宫颈癌患者血清miR-425-5p表达水平与总生存期的相关性。结果：与匹配的非癌组织相比，宫颈癌组织中miR-425-5p表达水平显着升高。 miR-425-5p 的较高表达与高肿瘤分期（P = 0.0003）和阳性淋巴结转移（P = 0.0107）呈正相关。与良性宫颈疾病和健康对照相比，宫颈癌患者的 miR-425-5p 血清浓度显着升高。此外，血清miR-425-5p的上调在TNM分期高（P = 0.0003）和淋巴结转移阳性（P = 0.0037）的宫颈癌患者中更常见。 Kaplan-Meier 分析显示，高血清 miR-425-5p 表达水平预示生存率较差 (P = 0.0571)。 Cox比例风险分析表明miR-425-5p是宫颈癌的独立预后因素。结论：我们的研究表明miR-425-5p在宫颈癌中表达上调，血清miR-425-5p可能作为宫颈癌潜在的预后生物标志物。
Background: MicroRNAs have been implicated in many biological pathways involved in tumourigenesis and can serve as prognostic biomarkers in many cancer types. The present study aims at evaluating the prognostic significance of miR-4255p in cervical cancer.Methods: Real-time polymerase chain reaction was performed to assess the expression levels of miR-425-5p in 35 pairs of cervical cancer tissues and their matched normal tissues as well as serum samples from 40 cervical cancer patients, 13 benign cervical disease patients and 32 healthy controls. The association between miR-425-5p expression levels in tissue and serum, and clinicopathological factors was examined. The correlation between serum miR-425-5p expression levels and overall survival of cervical cancer patients was assessed by Kaplan-Meier analysis and Cox proportional hazards model.Results: MiR-425-5p expression levels were significantly increased in cervical cancer tissues compared with matched non-cancerous tissues. Higher expression of miR-425-5p was positively associated with high tumour stage (P = 0.0003) and positive lymph node metastasis (P = 0.0107). Serum concentrations of miR-425-5p in cervical cancer patients were significantly higher compared with benign cervical disease and healthy controls. Moreover, the up-regulation of serum miR-425-5p occurred more frequently in cervical cancer patients with high TNM stage (P = 0.0003) and positive lymph node metastasis (P = 0.0037). Kaplan-Meier analysis showed that high serum miR-425-5p expression levels predicted poor survival (P = 0.0571). Cox proportional hazards risk analysis demonstrated that miR-425-5p was an independent prognostic factor for cervical cancer.Conclusion: Our study suggests that miR-425-5p is up-regulated in cervical cancer and serum miR-425-5p may serve as a potential prognostic biomarker for cervical cancer.