Role of cell-mediated immune reaction in blister formation of bullous pemphigoid.
Role of cell-mediated immune reaction in blister formation of bullous pemphigoid.
复制标题
细胞介导的免疫反应在大疱性类天疱疮形成水疱中的作用。
作者：
F. Kaneko;T. Minagawa;Y. Takiguchi;M. Suzuki;N. Itoh
在大疱性类天疱疮（BP）患者中，早期病变表现为渗出性斑块。组织学上，炎性浸润主要由单核细胞（MNC）组成，尽管也存在嗜酸性粒细胞和中性粒细胞。即使在大疱性病变中，MNC也主要是辅助/诱导T细胞。免疫组织化学染色显示部分浸润的MNCs胞浆内有抗人γ-干扰素（IFN-γ）单克隆抗体（MoAb）染色。这些浸润被认为产生IFN-γ。在早期BP病变的大疱液中，使用抗人IFN-γ单克隆抗体通过放射免疫测定法检测到高水平的IFN-γ。结果表明，浸润淋巴细胞在BP大疱性病变的免疫刺激。细胞介导的免疫反应以及基底膜区自身抗体也可能在BP水疱形成中起重要作用。
In patients with bullous pemphigoid (BP), early lesions appear as exudative erythematous patches. Histologically, the inflammatory infiltrate is composed mainly of mononuclear cells (MNCs) in the erythematous lesion, although eosinophils and neutrophils are also present. The MNCs are predominantly helper/inducer T cells even in the bullous lesions. Some of the MNCs infiltrated were stained in cytoplasm by antihuman gamma-interferon (IFN-gamma) monoclonal antibody (MoAb) immunohistologically. These infiltrates are considered to produce IFN-gamma. In the bullous fluids of early BP lesions, high levels of IFN-gamma are detected by radioimmunoassay using antihuman IFN-gamma MoAb. The results suggest that infiltrating lymphocytes are stimulated immunologically in BP bullous lesions. Cell-mediated immune reaction as well as autoantibody to the basement membrane zone may also play an important role in blister formation in BP.