Phenylketonuria: a new method for the simultaneous determination of plasma phenylalanine and tyrosine.
Phenylketonuria: a new method for the simultaneous determination of plasma phenylalanine and tyrosine.
复制标题
苯丙酮尿症：同时测定血浆苯丙氨酸和酪氨酸的新方法。
作者：
R. Shen;C. Abell
这种定量分光光度法基于苯丙氨酸解氨酶将苯丙氨酸和酪氨酸分别转化为反式肉桂酸和反式香豆酸。无需对样品进行脱蛋白或预先孵育，整个过程可在 20 分钟内完成。该方法对 1 微摩尔浓度的两种化合物敏感，仅需要 20 微升血浆或血清即可同时测定苯丙氨酸和酪氨酸。在健康个体的血清或血浆和苯丙酮尿症患者的血浆中，以0.1至40的摩尔比（苯丙氨酸与酪氨酸）测定这些氨基酸。
This quantitative spectrophotometric method is based on the conversion of phenylalanine and tyrosine by phenylalanine ammonia-lyase to trans-cinnamic acid and trans-coumaric acid, respectively. Neither deproteinization nor prior incubation of the sample is required, and the entire procedure can be performed in 20 minutes. The method is sensitive to 1-micromolar concentrations of the two compounds, and only 20 microliters of plasma or serum is required to determine both phenylalanine and tyrosine simultaneously. These amino acids were determined between molar ratios (phenylalanine to tyrosine) of 0.1 to 40 in the serum or plasma of healthy individuals and plasma of phenylketonuric patients.