Unusual Permeability of Porcine Endogenous Retrovirus Subgroup A Through Membrane Filters
Unusual Permeability of Porcine Endogenous Retrovirus Subgroup A Through Membrane Filters
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DOI：
10.1292/jvms.09-0184
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发表时间：
2010-01-01
影响因子：
1.2
通讯作者：
Miyazawa, Takayuki
中科院分区：
文献类型：
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作者：
Nakaya, Yuki;Shojima, Takayuki;Miyazawa, Takayuki
在从猪到人类的异种移植中，已经开发出一种生物人工内分泌胰腺（Bio-AEP），其中胰腺内分泌细胞被封装在孔径为100 nm的半透膜内。我们使用假型病毒 (LacZ(PERV-A)) 评估了猪内源性逆转录病毒 (PERV) 通过膜过滤器的渗透性，该假型病毒包含源自鼠白血病病毒的病毒核心和来自 PERV A 亚组的所有包膜 (Env)。与我们的预期相反，LacZ(PERV-A) 通过 200 nm 膜过滤器过滤而失去了感染性。在携带其他γ逆转录病毒包膜的假病毒中没有观察到这种不寻常的表型。通过反复冻融处理，LacZ(PERV-A)的感染性显着降低，表明LacZ(PERV-A)物理不稳定。此外，由于通过200 nm膜过滤，过滤后病毒RNA的拷贝数显着减少，因此LacZ(PERV-A)可能发生凝集。这种表型有利于开发安全的 Bio-AEP 来阻断 PI-RV 感染。
In xenotransplantation from pigs to humans, a bio-artificial endocrine pancreas (Bio-AEP), in which pancreatic endocrine cells are encapsulated within a semipermeable membrane of 100 nm pore size, has been developed. We evaluated the permeability of porcine endogenous retroviruses (PERVs) through membrane filters using a pseudotype virus (LacZ(PERV-A)) containing a viral core derived from murine leukemia virus and ail envelope (Env) from PERV Subgroup A. Contrary to our expectations, LacZ(PERV-A) lost its infectivity by filtration through a 200 nm membrane filter. This unusual phenotype was not observed in pseudotype Viruses harboring Envs from other gammaretroviruses. The infectivity of LacZ(PERV-A) was significantly decreased by repeated freeze/thaw treatment, indicating that LacZ(PERV-A) was physically labile. In addition, LacZ(PERV-A) may be agglutinated because copy numbers of viral RNA after filtration were significantly reduced by filtration through the 200 nm membrane. This phenotype is advantageous to develop a safe Bio-AEP blocking PI-RV infection.