DIFFERENTIAL EXPRESSION OF THROMBOSPONDIN-1, THROMBOSPONDIN-2, AND THROMBOSPONDIN-3 DURING MURINE DEVELOPMENT
DIFFERENTIAL EXPRESSION OF THROMBOSPONDIN-1, THROMBOSPONDIN-2, AND THROMBOSPONDIN-3 DURING MURINE DEVELOPMENT
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DOI：
10.1002/aja.1001970105
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发表时间：
1993-05-01
影响因子：
2.5
通讯作者：
BORNSTEIN, P
中科院分区：
文献类型：
--
作者：
IRUELAARISPE, ML;LISKA, DJ;BORNSTEIN, P
血小板反应蛋白1是一种分泌的三聚体糖蛋白，介导细胞与细胞外基质之间的相互作用，并对迁移和增殖表现出细胞特异性作用。最近，另外两个血小板反应蛋白基因（血小板反应蛋白2和3）已被确定。为了研究这些蛋白质的功能，我们使用原位杂交和RNA酶保护试验来比较小鼠胚胎发生过程中编码血小板反应蛋白1，2和3的基因的表达。血小板反应蛋白mRNA与骨化、神经器官发生和肺发育相关，尽管转录本的表达存在差异。从第10天到第13天，血小板反应蛋白1占主导地位。在此期间，在神经管、头部间充质和心脏垫中观察到高但短暂的表达水平。相比之下，一个更恒定的水平的血小板反应蛋白1的mRNA是明显的常驻巨核细胞的肝脏，以及在循环巨核细胞，既没有血小板反应蛋白2或3在这些细胞中检测。血小板反应蛋白1也由发育中的肾脏和肠道细胞产生。血小板反应蛋白2的表达主要局限于有组织的结缔组织，包括心包、胸膜、软骨膜、骨膜、脑膜、韧带和网状真皮。血小板反应蛋白2也是由分化的骨骼肌成肌细胞和肾脏和肠道细胞产生的。此外，在血管中检测到高水平的表达。血小板反应蛋白3 mRNA仅限于脑、软骨和肺。虽然血小板反应蛋白1，2和3属于一个家庭的结构相关的基因，在时空分布的相应的mRNA中观察到的差异表明这些分泌的蛋白质的独特功能。(C)1993 Wiley-Liss，Inc.
Thrombospondin 1 is a secreted, trimeric glycoprotein that mediates interactions between cells and extracellular matrix and exhibits cell-specific effects on migration and proliferation. Recently, two additional thrombospondin genes (thrombospondin 2 and 3) have been identified. To study the functions of these proteins, we have used in situ hybridization and RNAse protection assays to compare the expression of the genes encoding thrombospondin 1, 2, and 3 during murine embryogenesis. Thrombospondin mRNAs were associated with ossification, neuronal organogenesis, and lung development, although transcripts were differentially expressed. Thrombospondin 1 was predominant from days 10 to 13. During this period, high but transient levels of expression were observed in the neural tube, head mesenchyme, and cardiac cushions. In contrast, a more constant level of thrombospondin 1 mRNA was apparent in resident megakaryocytes of the liver, as well as in circulating megakaryocytes; neither thrombospondin 2 nor 3 was detected in these cells. Thrombospondin 1 was also produced by cells of the developing kidney and gut. The expression of thrombospondin 2 was confined principally to organized connective tissue that included pericardium, pleura, perichondrium, periosteum, meninges, ligaments, and reticular dermis. Thrombospondin 2 was also produced by differentiating skeletal myoblasts and by cells of the kidney and gut. Moreover, high levels of expression were detected in blood vessels. Thrombospondin 3 mRNA was restricted to brain, cartilage, and lung. Although thrombospondin 1, 2, and 3 belong to a family of structurally related genes, the differences observed in the spatiotemporal distribution of the corresponding mRNAs indicate unique functions for these secreted proteins. (C) 1993 Wiley-Liss, Inc.