ANISOMYCIN BLOCKS THE LATE PHASE OF LONG-TERM POTENTIATION IN THE DENTATE GYRUS OF FREELY MOVING RATS
ANISOMYCIN BLOCKS THE LATE PHASE OF LONG-TERM POTENTIATION IN THE DENTATE GYRUS OF FREELY MOVING RATS
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DOI：
10.1016/0361-9230(84)90005-4
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发表时间：
1984-01-01
影响因子：
3.8
通讯作者：
OTT, T
中科院分区：
文献类型：
--
作者：
KRUG, M;LOSSNER, B;OTT, T
在齿状回植入记录电极且在内侧内嗅皮层长期植入刺激电极的自由活动大鼠，在诱导单突触场电位长期增强（72小时）的强直刺激过程中，接受了两次脑室注射各400微克的茴香霉素。在强直刺激过程中抑制蛋白质合成并没有立即影响长时程增强（LTP）的诱导。然而，在强直刺激开始3 - 4小时后，增强效应逐渐减弱，并在剩余的7天观察期内完全消失。在对照实验中，茴香霉素不影响场兴奋性突触后电位（EPSP）的斜率，但会使群体峰电位幅度产生可逆性降低。显然，蛋白质合成抑制剂对相关机制有一种相对特异的作用，这种作用涉及LTP稳定的后期阶段。
Freely moving rats, chronically implanted with stimulation electrodes in the medial entorhinal cortex and recording electrodes in the dentate gyrus, received two 400 .mu.g intraventricular injections of anisomycin during a tetanization procedure that induced a long-lasting potentiation (72 h) of the monosynaptic field potential. Inhibition of protein synthesis during the tetanization procedure did not immediately influence the induction of long-term potentiation (LTP). However, 3-4 h after the beginning of tetanization the potentiation effect decayed progressively and was abolished totally during the remaining 7 day observation period. In control experiments anisomycin did not affect the slope of field EPSP [excitatory post-synaptic potentials] and produced a reversible depression of the population spike amplitude. Evidently, a relatively specific effect of the protein synthesis inhibitor on mechanisms is involved in a late phase of LTP stabilization.