Acousto-optical characterization of the viscoelastic nature of a nuchal elastin tissue scaffold.
Acousto-optical characterization of the viscoelastic nature of a nuchal elastin tissue scaffold.
复制标题
颈部弹性蛋白组织支架的粘弹性性质的声光表征。
DOI：
10.1089/107632703768247340
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Duncan,DonaldD
中科院分区：
文献类型：
--
作者：
Kirkpatrick,SeanJ;Hinds,MonicaT;Duncan,DonaldD
提出了一种非破坏性的，声光的方法来表征生物组织和组织支架的机械损耗因子，并应用于弹性蛋白组织支架的表征 来源于牛颈韧带。该方法依赖于用小型扬声器将引导表面声波发射到组织支架中，并同时照亮支架的一小部分区域 用低功率氦氖激光器在扬声器外照射。驱动声波与背散射激光散斑图案中的移位之间的相位滞后被确定为机械特性的量度。 支架的损耗因子tanδ。还通过传统的动态机械加载技术进行tanδ和弹性模量的测量。通过中心部分的 加载循环后，弹性蛋白支架的弹性模量为1.2 × 106± 1 × 105 N· m-2（平行于纤维方向）。估计值 平行于弹性纤维方向的tanδ值为0.03 ± 0.017，声光法为0.029 ± 0.03。垂直的方向上 对于纤维取向，声光法测得tanδ为0.14 ± 0.056。由于缺乏机械完整性，无法测量 垂直于纤维取向的方向。声光方法可能被证明是有用的力学特性的开发工程组织。
A nondestructive, acousto-optical method for characterizing the mechanical loss factor of biological tissues and tissue scaffolds is presented and applied to the characterization of an elastin tissue scaffold derived from bovine nuchal ligament. The method relies on launching guided surface acoustic waves into the tissue scaffold with a small speaker and simultaneously illuminating a small region of the scaffold distant from the speaker with a low-power HeNe laser. The phase lag between the driving acoustic wave and the shift in the backscattered laser speckle pattern is determined as a measure of the mechanical loss factor of the scaffold, tanδ. Measurements of tanδand elastic modulus were also made by traditional dynamic mechanical loading techniques. Through the central portion of the loading cycle, the elastic modulus of the elastin scaffold was 1.2 × 106± 1 × 105N · m-2(parallel to fiber orientation). The estimated value of tanδin the direction parallel to the elastin fibers was 0.03 ± 0.017 by traditional methods and 0.029 ± 0.03 when using the acousto-optical method. In the direction perpendicular to fiber orientation, tanδwas measured as 0.14 ± 0.056 by the acousto-optical method. Because of a lack of mechanical integrity, it was not possible to measure tanδin the direction perpendicular to fiber orientation by traditional methods. The acousto-optical method may prove to be useful in the mechanical characterization of developing engineered tissues.